ID   CHS4_BROFI              Reviewed;         394 AA.
AC   O23730;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Chalcone synthase 4;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase 4;
GN   Name=CHS4;
OS   Bromheadia finlaysoniana (Orchid).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Orchidaceae;
OC   Epidendroideae; Vandeae; Adrorhizinae; Bromheadia.
OX   NCBI_TaxID=41205;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Flower;
RA   Liew C.F., Lim S.H., Loh C.S., Goh C.J.;
RT   "Molecular cloning and sequence analysis of chalcone synthase cDNAs of
RT   Bromheadia finlaysoniana.";
RL   Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; AF007098; AAB62875.1; -; mRNA.
DR   AlphaFoldDB; O23730; -.
DR   SMR; O23730; -.
DR   PRIDE; O23730; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..394
FT                   /note="Chalcone synthase 4"
FT                   /id="PRO_0000215957"
FT   ACT_SITE        165
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   394 AA;  42915 MW;  3E6CE4BEA980BBB2 CRC64;
     MAPAMEEIRQ AQRAEGPAAV LAIGTSTPPN ALYQADYPDY YFRITKSEHL TELKEKFKRM
     CDKSMIKKRY MYLTEEILKE NPNICAFMAP SLDARQDIVV TEVPKLAKEA AVRAIKEWGH
     PKSRITHLIF CTTSGIDMPG ADYQLTRLLG LRPSVNRFML YQQGCFAGGT VLRLAKDLAE
     NNAGARVLVV CSEITAVTFR GPSESHLDSL VGQALFGDGA AAIIVGSDPD SATERPLFQL
     VSASQTILPE SEGAIDGHLR EIGLTFHLLK DVPGLISKNI QKCLLDAFKP LGVHDWNSIF
     WIAHPGGPAI LDQVEIKLGL KAEKLAASRS VLAEYGNMSS ACVLFILDEM RRRSAEAGQA
     TTGEGLEWGV LFGFGPGLTV ETIVLRSVPI AGAE