ID   CHS4_SORBI              Reviewed;         401 AA.
AC   Q9SBL5;
DT   27-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=Chalcone synthase 4;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase 4;
GN   Name=CHS4;
OS   Sorghum bicolor (Sorghum) (Sorghum vulgare).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Sorghinae; Sorghum.
OX   NCBI_TaxID=4558;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. BTx623;
RA   Lo S.-C.C., Nicholson R.L.;
RT   "Molecular cloning of chalcone synthase genes from sorghum.";
RL   Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; AF152551; AAD41876.1; -; Genomic_DNA.
DR   RefSeq; XP_002450870.1; XM_002450825.1.
DR   AlphaFoldDB; Q9SBL5; -.
DR   SMR; Q9SBL5; -.
DR   STRING; 4558.Sb05g020160.1; -.
DR   EnsemblPlants; EES09858; EES09858; SORBI_3005G136300.
DR   GeneID; 8056976; -.
DR   Gramene; EES09858; EES09858; SORBI_3005G136300.
DR   KEGG; sbi:8056976; -.
DR   eggNOG; ENOG502QRSY; Eukaryota.
DR   HOGENOM; CLU_034992_2_0_1; -.
DR   OMA; NFMGCYA; -.
DR   OrthoDB; 950070at2759; -.
DR   UniPathway; UPA00154; -.
DR   ExpressionAtlas; Q9SBL5; baseline and differential.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..401
FT                   /note="Chalcone synthase 4"
FT                   /id="PRO_0000216058"
FT   ACT_SITE        168
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   401 AA;  43669 MW;  55D52AF3717326C9 CRC64;
     MAAATVTVEE VRKAQRATGP ATVLAIGTAT PANCVHQADY PDYYFRITKS EHMTELKEKF
     KRMCDKSQIR KRYMHLTEEY LAENPNMCAY MAPSLDARQD IVVVEVPKLG KAAAQKAIKE
     WGQPKSKITH LVFCTTSGVD MPGADYQLTK MLGLRPSVKR LMMYQQGCFA GGTVLRVAKD
     LAENNRGARV LVVCSEITAV TFRGPSESHL DSMVGQALFG DGAAAVIVGA DPDERVERPL
     FQLVSASQTI LPDSEGAIDG HLREVGLTFH LLKDVPGLIS KNIERSLEEA FKPLGITDYN
     SIFWVAHPGG PAILDQVEAK VGLKKERMRA TRHVLSEYGN MSSACVLFIL DEMRKRSAED
     GQATTGEGLD WGVLFGFGPG LTVETVVLHS VPITTGAAIT A