ID   CHS5_MEDSA              Reviewed;         389 AA.
AC   P51078;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Chalcone synthase 4-2;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase 4-2;
GN   Name=CHS4-2; Synonyms=CHSI;
OS   Medicago sativa (Alfalfa).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX   NCBI_TaxID=3879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Nagyszenasi; TISSUE=Leaf;
RX   PubMed=8274775; DOI=10.1094/mpmi-6-655;
RA   Esnault R., Buffard D., Breda C., Sallaud C., El-Turk J., Kondorosi A.;
RT   "Pathological and molecular characterizations of alfalfa interactions with
RT   compatible and incompatible bacteria, Xanthomonas campestris pv. alfalfae
RT   and Pseudomonas syringae pv. pisi.";
RL   Mol. Plant Microbe Interact. 6:655-664(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 19-389.
RC   STRAIN=cv. Iroquois; TISSUE=Root nodule;
RX   PubMed=8193301; DOI=10.1007/bf00029858;
RA   McKhann H.I., Hirsch A.M.;
RT   "Isolation of chalcone synthase and chalcone isomerase cDNAs from alfalfa
RT   (Medicago sativa L.): highest transcript levels occur in young roots and
RT   root tips.";
RL   Plant Mol. Biol. 24:767-777(1994).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- DEVELOPMENTAL STAGE: Highest expression in young root tips.
CC   -!- INDUCTION: By infection.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X68106; CAA48226.1; -; mRNA.
DR   EMBL; U01019; AAB41560.1; -; mRNA.
DR   PIR; S26414; S26414.
DR   AlphaFoldDB; P51078; -.
DR   SMR; P51078; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..389
FT                   /note="Chalcone synthase 4-2"
FT                   /id="PRO_0000216010"
FT   ACT_SITE        164
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
FT   CONFLICT        86
FT                   /note="I -> Y (in Ref. 2; AAB41560)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        94
FT                   /note="W -> R (in Ref. 2; AAB41560)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   389 AA;  42661 MW;  B33674EC7709F170 CRC64;
     MVSVSEIRNA QRAEGPATTL AIGTANPTNC VEQSTYPDFY FKITNSEHKT ELKEKFQRMC
     DKSMIKRRYM YLTEEILKEN PSVCEIMAPS LDAWQDMVVV EVPRLGKEAA VKAIKEWGQP
     KSKITHLIVC TTSGVDMPGA DYQLTKLLGL RPYVKRYMMY QQGCFAGGTV LRLAKDLAEN
     NKGARVLVVC SEVTAVTFRG PSDTHLDSLV GQALFGDGAA ALIVGSDPVP EIEKPIFEMV
     WTAQTIAPDS EGAIDGHLRE AGLTFHLLKD VPGIVSKNIN KALVEAFEPL GISDYNSIFW
     IAHPGGPAIL DQVEQKLALK PEKMKATREV LSEYGNMSSA CVLFILDEMR KKSAQDGLKT
     TGEGLEWGVL FGFGPGLTIE TVVLRSVAI