ID   CHS6_MEDSA              Reviewed;         285 AA.
AC   P51079;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Chalcone synthase 6-4;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase 6-4;
DE   Flags: Fragment;
GN   Name=CHS6-4;
OS   Medicago sativa (Alfalfa).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX   NCBI_TaxID=3879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Iroquois; TISSUE=Root nodule;
RX   PubMed=8193301; DOI=10.1007/bf00029858;
RA   McKhann H.I., Hirsch A.M.;
RT   "Isolation of chalcone synthase and chalcone isomerase cDNAs from alfalfa
RT   (Medicago sativa L.): highest transcript levels occur in young roots and
RT   root tips.";
RL   Plant Mol. Biol. 24:767-777(1994).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- DEVELOPMENTAL STAGE: Highest expression in young root tips.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; U01020; AAB41558.1; -; mRNA.
DR   PIR; S44369; S44369.
DR   AlphaFoldDB; P51079; -.
DR   SMR; P51079; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   SUPFAM; SSF53901; SSF53901; 1.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           <1..285
FT                   /note="Chalcone synthase 6-4"
FT                   /id="PRO_0000216011"
FT   ACT_SITE        60
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
FT   NON_TER         1
SQ   SEQUENCE   285 AA;  30784 MW;  76C2C0D02AA81FF3 CRC64;
     LGKEAAVKAI KEWGQPKSKI THLIFCTTSG VDMPGADYQL TKLLGLRPYV KRYMMYQQGC
     FAGGTVLRLA KDLAENNKGA RVLVVCSEVT AVTFRGPSDT HLDSLVGQAL FGDGAAALIV
     GSDPIPEIEK PIFEMVWTAQ TIAPDSEGAI DGHLREAGLT FHLLKDVPGI VSKNIDKALV
     EAFQPLNISD YNSIFWIAHP GGPAILDQVE QKLGLKPEKM KATREVLSEY GNMSSACVLF
     ILDEMRKKSA QQGLKTTGEG LDWGVLFGFG PGLTIETVVL HSVAL