ID   CHS7_PICMA              Reviewed;         395 AA.
AC   Q9M5M0;
DT   08-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Chalcone synthase 7;
DE            EC=2.3.1.74;
DE   AltName: Full=Naregenin-chalcone synthase 7;
GN   Name=CSF7;
OS   Picea mariana (Black spruce) (Abies mariana).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Picea.
OX   NCBI_TaxID=3335;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Cone;
RA   Xue B.G., Rutledge R.G.;
RT   "Characterization of the black spruce chalcone gene family.";
RL   Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; AF227627; AAF35890.1; -; mRNA.
DR   AlphaFoldDB; Q9M5M0; -.
DR   SMR; Q9M5M0; -.
DR   PRIDE; Q9M5M0; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..395
FT                   /note="Chalcone synthase 7"
FT                   /id="PRO_0000216040"
FT   ACT_SITE        169
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   395 AA;  43175 MW;  622A09497800ADED CRC64;
     MAGGLMADLE AFRKAQRADG PATILAIGTA TPPNAVDQST YPDYYFKITN SEHMTELKEK
     FQRMCDKSAI KKRYMYLTDE ILKENPNVCE YMAPSLDARQ DMVVVEVPRL GKEAATKAIK
     EWGQPKSKIT HVIFCTTSGV DMPGADYQLT KLLGLRPSVK RVMMYQQGCF AGGTVLRVAK
     DLAENNRGAR VLVVCSEITA VTFRGPSDTH LDSMVGQALF GDGAAALIVG ADPIPQVEKP
     CFELMWTAQT ILPDSDGAID GHLREVGLTF HLLKDVPGLI SKNIEKSLVE AFQQFGISDW
     NQLFWIAHPG GPAILDQVEA KLNLDPKKLR ATRQVLSEYG NMSSACVHFI LDEMRKSSNE
     KGCSTTGEGL DVGVLFGFGP GLTVETVVLK SVPLQ