ID   CHS9_DAUCA              Reviewed;         389 AA.
AC   Q9SB26;
DT   21-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Chalcone synthase 9;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase 9;
GN   Name=CHS9;
OS   Daucus carota (Wild carrot).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Apiales; Apiaceae; Apioideae; Scandiceae; Daucinae;
OC   Daucus; Daucus sect. Daucus.
OX   NCBI_TaxID=4039;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Kurodagosun;
RA   Ozeki Y., Davies E., Takeda J.;
RT   "Structure and expression of chalcone synthase gene in carrot suspension
RT   cultured cells regulated by 2,4-D.";
RL   Plant Cell Physiol. 34:1029-1037(1993).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; D16256; BAA03785.1; -; mRNA.
DR   AlphaFoldDB; Q9SB26; -.
DR   SMR; Q9SB26; -.
DR   PRIDE; Q9SB26; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..389
FT                   /note="Chalcone synthase 9"
FT                   /id="PRO_0000215972"
FT   ACT_SITE        164
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   389 AA;  42711 MW;  50EC12B35FDD55F0 CRC64;
     MVTVNEFRKA QRAEGPATVL AIGTATPPNC VDQSAYADYY FRITNSEDKP ELKEKFRRMC
     EKSMINTRYM HLTEDLLKQN PSFCEYMASS LDARQDIVVN EVPKLGKEAA LRAIKEWGRP
     KSKITHLIFC TTSGVDMPGA DFRLTKLLGL RPSVKRFMMY QQGCFAGGTV LRLAKDLAEN
     NKNARVLVVC SEITVITFRG PNDTHLDSLV GQALFGDGAG AVIVGSDPVI GIEKPLFEIV
     SAAQTILPDS DGAIDGHLRE VGLTFHLLKD VPGLISKNIR KSLVEAFKPL GISDWNSIFW
     IAHPGGPAIL DQVETELSLK PDKLKSTRQV LRDYGNMSSA CVLFILDEMR NASAKDGHRT
     TGEGLDWGVL FGFGPGLTVE TVVLHSVPT