CHS9_MEDSA
ID CHS9_MEDSA Reviewed; 389 AA.
AC P30077;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 1.
DT 25-MAY-2022, entry version 86.
DE RecName: Full=Chalcone synthase 9;
DE EC=2.3.1.74;
DE AltName: Full=Naringenin-chalcone synthase 9;
GN Name=CHS9;
OS Medicago sativa (Alfalfa).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3879;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8507827; DOI=10.1007/bf00014932;
RA Junghans H., Dalkin K., Dixon R.A.;
RT "Stress responses in alfalfa (Medicago sativa L.). 15. Characterization and
RT expression patterns of members of a subset of the chalcone synthase
RT multigene family.";
RL Plant Mol. Biol. 22:239-253(1993).
CC -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC which can under specific conditions spontaneously isomerize into
CC naringenin.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000305}.
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DR EMBL; L02905; AAA02827.1; -; mRNA.
DR PIR; S35167; S35167.
DR AlphaFoldDB; P30077; -.
DR SMR; P30077; -.
DR UniPathway; UPA00154; -.
DR GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR InterPro; IPR011141; Polyketide_synthase_type-III.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR11877; PTHR11877; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF00195; Chal_sti_synt_N; 1.
DR PIRSF; PIRSF000451; PKS_III; 1.
DR SUPFAM; SSF53901; SSF53901; 2.
DR PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Flavonoid biosynthesis; Transferase.
FT CHAIN 1..389
FT /note="Chalcone synthase 9"
FT /id="PRO_0000216014"
FT ACT_SITE 164
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ SEQUENCE 389 AA; 42721 MW; BB461B53E8C63002 CRC64;
MVSVSEIRQA QRAEGPATIM AIGTANPANC VEQSTYPDFY FKITNSEHKV ELKEKFQRMC
DKSMIKRRYM YLTEEILKEN PSVCEYMAPS LDARQDMVVV EVPRLGKEAA VKAIKEWGQP
KSKITHLIFC TTSGVDMPGA DYQLTKLLGL RPYVKRYMMY QQGCFAGGTV LRLAKDLAEN
NKGARVLVVC SEVTAVTFRG PSDTHLDSLV GQALFGDGAA ALIVGSDPIP EIEKPIFEMV
WTAQTIAPDS EGAIDGHLRE AGLTFHLLKD VPGIVSKNID KALVEAFQPL NISDYNSIFW
IAHPGGPAIL DQVEQKLGLK PEKMKATREV LSEYGNMSSA CVLFILDEMR KKSAQAGLKT
TGEGLDWGVL FGFGPGLTIE TVVLHSVAI