ID   CHSA_IPOPU              Reviewed;         396 AA.
AC   P48397; O04984;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1999, sequence version 2.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Chalcone synthase A;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase A;
DE            Short=CHS-A;
GN   Name=CHSA;
OS   Ipomoea purpurea (Common morning glory) (Pharbitis purpurea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Convolvulaceae; Ipomoeeae; Ipomoea.
OX   NCBI_TaxID=4121;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7724563; DOI=10.1073/pnas.92.8.3338;
RA   Durbin M.L., Learn G.H., Huttley G.A., Clegg M.T.;
RT   "Evolution of the chalcone synthase gene family in the genus Ipomoea.";
RL   Proc. Natl. Acad. Sci. U.S.A. 92:3338-3342(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=IMEX16, IMEX19, IMEX22, and IMEX8;
RA   Huttley G.A., Durbin M.L., Glover D.E., Clegg M.T.;
RT   "Nucleotide polymorphism in the chalcone synthase-A locus and evolution of
RT   the chalcone synthase multigene family of common morning glory (Ipomoea
RT   purpurea).";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; U15946; AAB02620.1; -; Genomic_DNA.
DR   EMBL; U84905; AAB62582.1; -; Genomic_DNA.
DR   EMBL; U84907; AAB62584.1; -; Genomic_DNA.
DR   EMBL; U84908; AAB62585.1; -; Genomic_DNA.
DR   EMBL; U84909; AAB62586.1; -; Genomic_DNA.
DR   PIR; T10958; T10958.
DR   AlphaFoldDB; P48397; -.
DR   SMR; P48397; -.
DR   PRIDE; P48397; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..396
FT                   /note="Chalcone synthase A"
FT                   /id="PRO_0000215992"
FT   ACT_SITE        170
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
FT   CONFLICT        14..19
FT                   /note="HRAKRL -> RREKRN (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        114
FT                   /note="D -> E (in Ref. 1; AAB02620)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        190
FT                   /note="G -> A (in Ref. 1; AAB02620)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        225
FT                   /note="A -> S (in Ref. 1; AAB02620)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        285
FT                   /note="V -> I (in Ref. 1; AAB02620)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        362..363
FT                   /note="DE -> QQ (in Ref. 1; AAB02620)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   396 AA;  43805 MW;  2C06EA046DFCAC7C CRC64;
     MSTTVTVLTD TWSHRAKRLE GDAKILAIGT ATPANWVDQT TYPDFYFRIT NSEHLLEHKE
     KFRRICNKSK IMKRHLVITE ELLKKNPNLC TYNEASLNTR QDILVSEVPK LGKDAAMKAI
     KEWGRPISEI THLVFCTTSG VDMPGADFQL TKLLGLNSSV KRLMMYQQGC NAGAAMLRLA
     KDLAENNKGG RVLVVCSEVM LSVFRGPSLQ QEDNLLAQCL FGDGAAAVIV GTEPRPGLET
     PLFELVSAAQ TTIPDTDSYL KLQLREMGLT FHCSKAVPSL ITQNVEDCLV KAFEPFGISD
     WNSIFWILHP GGNAILDGVE EKLGLEPEKL RASRDVLSQY GNLTSACVLF ILDEVRKKSK
     KDEQMTTGEG LEWGVVFGFG PGLTIDTVVI RSVPIN