ID   CHSA_PETHY              Reviewed;         389 AA.
AC   P08894;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Chalcone synthase A;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase A;
GN   Name=CHSA;
OS   Petunia hybrida (Petunia).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Petunioideae; Petunia.
OX   NCBI_TaxID=4102;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Violet 30;
RX   PubMed=3016642; DOI=10.1093/nar/14.13.5229;
RA   Koes R.E., Spelt C.E., Reif H.J., van den Elzen P.J.M., Veltkamp E.,
RA   Mol J.N.M.;
RT   "Floral tissue of Petunia hybrida (V30) expresses only one member of the
RT   chalcone synthase multigene family.";
RL   Nucleic Acids Res. 14:5229-5239(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Violet 30; TISSUE=Leaf;
RX   PubMed=2806915; DOI=10.1016/0378-1119(89)90185-6;
RA   Koes R.E., Spelt C.E., van den Elzen P.J.M., Mol J.N.M.;
RT   "Cloning and molecular characterization of the chalcone synthase multigene
RT   family of Petunia hybrida.";
RL   Gene 81:245-257(1989).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- TISSUE SPECIFICITY: Major expressed member of the gene family in
CC       various floral tissues and in seedlings treated with UV light. It is
CC       relatively low expressed in tissue culture material.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; X04080; CAA27718.1; -; mRNA.
DR   EMBL; X14591; CAA32731.1; -; Genomic_DNA.
DR   PIR; A23643; SYPJCN.
DR   PIR; JS0308; SYPJCA.
DR   AlphaFoldDB; P08894; -.
DR   SMR; P08894; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..389
FT                   /note="Chalcone synthase A"
FT                   /id="PRO_0000216029"
FT   ACT_SITE        164
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
FT   CONFLICT        128
FT                   /note="V -> F (in Ref. 2; CAA32731)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   389 AA;  42525 MW;  0221B8DA1E96EAF6 CRC64;
     MVTVEEYRKA QRAEGPATVM AIGTATPTNC VDQSTYPDYY FRITNSEHKT DLKEKFKRMC
     EKSMIKKRYM HLTEEILKEN PSMCEYMAPS LDARQDIVVV EVPKLGKEAA QKAIKEWGQP
     KSKITHLVFC TTSGVDMPGC DYQLTKLLGL RPSVKRLMMY QQGCFAGGTV LRLAKDLAEN
     NKGARVLVVC SEITAVTFRG PNDTHLDSLV GQALFGDGAG AIIIGSDPIP GVERPLFELV
     SAAQTLLPDS HGAIDGHLRE VGLTFHLLKD VPGLISKNIE KSLEEAFKPL GISDWNSLFW
     IAHPGGPAIL DQVEIKLGLK PEKLKATRNV LSDYGNMSSA CVLFILDEMR KASAKEGLGT
     TGEGLEWGVL FGFGPGLTVE TVVLHSVAT