ID   CHSB_IPONI              Reviewed;         363 AA.
AC   P48396;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Chalcone synthase B;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase B;
DE            Short=CHS-B;
DE   Flags: Fragment;
GN   Name=CHSB;
OS   Ipomoea nil (Japanese morning glory) (Pharbitis nil).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Convolvulaceae; Ipomoeeae; Ipomoea.
OX   NCBI_TaxID=35883;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7724563; DOI=10.1073/pnas.92.8.3338;
RA   Durbin M.L., Learn G.H., Huttley G.A., Clegg M.T.;
RT   "Evolution of the chalcone synthase gene family in the genus Ipomoea.";
RL   Proc. Natl. Acad. Sci. U.S.A. 92:3338-3342(1995).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; U15944; AAC49029.1; -; Genomic_DNA.
DR   AlphaFoldDB; P48396; -.
DR   SMR; P48396; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..>363
FT                   /note="Chalcone synthase B"
FT                   /id="PRO_0000216036"
FT   ACT_SITE        170
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
FT   NON_TER         363
SQ   SEQUENCE   363 AA;  40484 MW;  9349FABA60C7C3FA CRC64;
     MSTILNVLTD TWSPRAKKLE GDAKIWAIGT ATPANWVDQT TYPDFYFRIT NSQHLLEHKE
     KFRRICNKSK IRKRHLVLTE ELLKKNPNLC TYNETSLNTR QDILVAEVPK LGKEAAMKAI
     KEWGRPISEI THLVFCTTSG VDMPGADFQL TKLLGLNSSV KRLMMYQQGC NAGAAMLRLA
     KDLAESNKGG RVLVVCAEIT INIFRGPSLE QDDNLLAQCL FGDGAAAMIV AADPRPGLET
     PLFELVSSAQ TIVPNTDSHL KLHLREMGLT FHCSKAVPSV LAENVEDCLV KAFEPYGISD
     WNSIFWVFHP GGNAIVDRVE ERLGLGPEKF RASRDVLSEY GNLTSACVLF TLDEMRKKSK
     KDE