ID   CHSB_IPOPU              Reviewed;         396 AA.
AC   P48398;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Chalcone synthase B;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase B;
DE            Short=CHS-B;
GN   Name=CHSB;
OS   Ipomoea purpurea (Common morning glory) (Pharbitis purpurea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Convolvulaceae; Ipomoeeae; Ipomoea.
OX   NCBI_TaxID=4121;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7724563; DOI=10.1073/pnas.92.8.3338;
RA   Durbin M.L., Learn G.H., Huttley G.A., Clegg M.T.;
RT   "Evolution of the chalcone synthase gene family in the genus Ipomoea.";
RL   Proc. Natl. Acad. Sci. U.S.A. 92:3338-3342(1995).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; U15947; AAC49030.1; -; Genomic_DNA.
DR   PIR; T10951; T10951.
DR   AlphaFoldDB; P48398; -.
DR   SMR; P48398; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..396
FT                   /note="Chalcone synthase B"
FT                   /id="PRO_0000215993"
FT   ACT_SITE        170
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   396 AA;  43927 MW;  352C478C1AF892B6 CRC64;
     MSTTVTVLTD TWSRRAKRLE GDAKILAIGT ATPASWVDQT TYPDFYFRIT NSQHLLEHKE
     KFRRICNKSK IRKRHLVLTE ELLKKNPNLC TYNETSLNTR QDILVSEVPK LGKEAAMKAI
     KEWGRPISEI THLVFCTTSG VDMPGADFQL TKLLGLNSSV KRLMMYQQGC NAGAAMLRLA
     KDLAESNKGG RVLVVCAEIT INIFRGPSLE QDDNLLAQCL FGDGAAAMIV AADPRPGLET
     PLFELVSSAQ TIVPNTDSHL KLHLREMGLT FHCSKAVPSV LAENVEDCLV KAFEPYGISD
     WNSIFWVFHP GGNAIVDRVE ERLGLGPEKL RASRDVLSEY GNLTSACVLF ILDEMRKKSK
     KDEQMTTGEG LEWGVVFGFG PGLTIDTIII RSVPIN