ID   CHSB_IPOTF              Reviewed;         366 AA.
AC   P48402;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Chalcone synthase B;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase B;
DE            Short=CHS-B;
DE   Flags: Fragment;
GN   Name=CHSB;
OS   Ipomoea trifida (Morning glory).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Convolvulaceae; Ipomoeeae; Ipomoea.
OX   NCBI_TaxID=35884;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7724563; DOI=10.1073/pnas.92.8.3338;
RA   Durbin M.L., Learn G.H., Huttley G.A., Clegg M.T.;
RT   "Evolution of the chalcone synthase gene family in the genus Ipomoea.";
RL   Proc. Natl. Acad. Sci. U.S.A. 92:3338-3342(1995).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; U15951; AAC49033.1; -; Genomic_DNA.
DR   AlphaFoldDB; P48402; -.
DR   SMR; P48402; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..>366
FT                   /note="Chalcone synthase B"
FT                   /id="PRO_0000215998"
FT   ACT_SITE        172
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
FT   NON_TER         366
SQ   SEQUENCE   366 AA;  40808 MW;  33448EB9177F775D CRC64;
     MSTAVTMLTD TWSRRAKRFE IEGYAKILAI GTATPANWVD QTTYPDFYFR ITNSQHLLEH
     KEKFRRICNK SNIRKRHMVL TEELLKKNPN LCTYNDASLN TRQDILVSEV PKLGKEAAMK
     AIKVWGRPIS EITHLVFCTT SGVDMPGADF QLTKLLGLNS SVKRLMMYQQ GCNAGAAMLR
     LAKDLAENNK GARVLVVCSE VMLSVFRGPS LQQEDNLLAQ CLFGDGSAAI IIGTDPRPGL
     ETPLFELISA AQTIIPDTDS HLKLHVREMG LTFHCSKAVP TFITQNVEDC LVKAFEPYGI
     SDWNSIFWVL HPGGNAIVEG VEETLGLAPE KLRASRDVLS EYGNLTSACV LFILDEVRKK
     SKKDEQ