ID   CHSB_PEA                Reviewed;         389 AA.
AC   P51082;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Chalcone synthase 1B;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase 1B;
GN   Name=CHS-1B;
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. JI 813;
RA   Hellens R.P.;
RL   Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; X80007; CAA56317.1; -; Genomic_DNA.
DR   PIR; S49203; S49203.
DR   AlphaFoldDB; P51082; -.
DR   SMR; P51082; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..389
FT                   /note="Chalcone synthase 1B"
FT                   /id="PRO_0000216025"
FT   ACT_SITE        164
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   389 AA;  42821 MW;  963105A8C4010C52 CRC64;
     MVTVDEIRQA QKAEGPATVL AIGTATPPNC VDQSTYPDYY FRITNSEHKT ELKEKFQRMC
     DKSMIKKRYM HLTEEILKEN PSVCEYMAPS LDARQDMVVV EVPKLGKEAA TKAIKEWGQP
     KSKITHLIFC TTSGVDMPGA DYQLTKLLGL RPHVKRYMMY QQGCFAGGTV LRLAKDLAEN
     NKGARVLVVC SEITAVTFRG PSDTHLDSLV GQALFGDGAA AVIVGSDPLP QVEKPLFELV
     WTAQTILPDS EGAIDGHLRE VGLTFHLLKD VPGLISKNIE KALVEAFQPL GISDYNSLFW
     IAHPGGPAIL DQVEAKLSLK QRKMQATRHV LSEYGNMSSA CVLFILDEMR RKSKEEQLGT
     TGEGLEWGVL FGFGPGLTVE TVVLHSVAT