ID   CHSE_IPONI              Reviewed;         389 AA.
AC   O22046;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Chalcone synthase E;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase E;
DE            Short=CHS-E;
GN   Name=CHSE;
OS   Ipomoea nil (Japanese morning glory) (Pharbitis nil).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Convolvulaceae; Ipomoeeae; Ipomoea.
OX   NCBI_TaxID=35883;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. KK/ZSK-2; TISSUE=Flower bud;
RX   PubMed=9249990; DOI=10.1093/oxfordjournals.pcp.a029232;
RA   Fukada-Tanaka S., Hoshino A., Hisatomi Y., Habu Y., Hasebe M., Iida S.;
RT   "Identification of new chalcone synthase genes for flower pigmentation in
RT   the Japanese and common morning glories.";
RL   Plant Cell Physiol. 38:754-758(1997).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; AB001819; BAA21788.1; -; mRNA.
DR   RefSeq; XP_019165608.1; XM_019310063.1.
DR   AlphaFoldDB; O22046; -.
DR   SMR; O22046; -.
DR   GeneID; 109161594; -.
DR   KEGG; ini:109161594; -.
DR   OrthoDB; 950070at2759; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..389
FT                   /note="Chalcone synthase E"
FT                   /id="PRO_0000216038"
FT   ACT_SITE        164
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   389 AA;  42685 MW;  0FC0E014B9CC0312 CRC64;
     MVTVEEVRKA QRAQGPATIM AIGTSTPQNC VDQSTYPDYY FRITNSEHLV ELKEKFKRMC
     EKSMIKKRYM YLTEEILKEN PNICAYMAPS LDARQDIVVV EVPKLGKEAA QKAIKEWGQP
     KSKITHLVFC TTSGVDMPGA DYQLTKLLGL QPSVKRFMMY QQGCFAGGTV IRLAKDLAEN
     NKGARVLVVC SEITAVTFRG PSDAHLDSLV GQALFGDGAA ALIIGSDPDP DLERPLFQLV
     SAAQTILPDS GGAIDGHLRE VGLTFHLLKD VPGLISKHIE KSLNEAFQPL GIHDWNSLFW
     IAHPGGPAIL DQVEEKLELK PEKLRATRHV LSEYGNMSSA CVLFILDEMR KASSKEGLNT
     TGEGLEWGVL FGFGPGLTVE TVVLHSVSA