CHSH2_HUMLU
ID CHSH2_HUMLU Reviewed; 389 AA.
AC A0AMG7;
DT 02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2006, sequence version 1.
DT 25-MAY-2022, entry version 62.
DE RecName: Full=Chalcone synthase H2 {ECO:0000305};
DE Short=CHS_H1-1539 {ECO:0000303|PubMed:17002429};
DE EC=2.3.1.74 {ECO:0000255|PROSITE-ProRule:PRU10023};
DE AltName: Full=Naringenin-chalcone synthase CHS_H1-1539 {ECO:0000303|PubMed:17002429};
GN Name=chs_H1-1539 {ECO:0000303|PubMed:17002429};
OS Humulus lupulus (European hop).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Cannabaceae; Humulus.
OX NCBI_TaxID=3486;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND GENE FAMILY.
RC STRAIN=cv. Osvals's 72; TISSUE=Leaf;
RX PubMed=17002429; DOI=10.1021/jf061785g;
RA Matousek J., Vrba L., Skopek J., Orctova L., Pesina K., Heyerick A.,
RA Baulcombe D., De Keukeleire D.;
RT "Sequence analysis of a 'true' chalcone synthase (chs_H1) oligofamily from
RT hop (Humulus lupulus L.) and PAP1 activation of chs_H1 in heterologous
RT systems.";
RL J. Agric. Food Chem. 54:7606-7615(2006).
RN [2]
RP PATHWAY, AND REVIEW.
RX PubMed=30468448; DOI=10.1039/c8np00077h;
RA Liu Y., Jing S.-X., Luo S.-H., Li S.-H.;
RT "Non-volatile natural products in plant glandular trichomes: chemistry,
RT biological activities and biosynthesis.";
RL Nat. Prod. Rep. 36:626-665(2019).
CC -!- FUNCTION: Involved in the biosynthesis of prenylated phenolics natural
CC products which contribute to the bitter taste of beer and display broad
CC biological activities (Probable). Chalcone synthase that can use 4-
CC coumaroyl-CoA to produce 4,2',4',6'-tetrahydroxychalcone (also termed
CC naringenin-chalcone or chalcone) which can, under specific conditions,
CC spontaneously isomerize into naringenin (By similarity).
CC {ECO:0000250|UniProtKB:Q9FEY5, ECO:0000305|PubMed:30468448}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC {ECO:0000305|PubMed:30468448}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9FEY5}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000305}.
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DR EMBL; AM263199; CAK19317.1; -; Genomic_DNA.
DR AlphaFoldDB; A0AMG7; -.
DR SMR; A0AMG7; -.
DR BRENDA; 2.3.1.74; 2716.
DR UniPathway; UPA00154; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR InterPro; IPR011141; Polyketide_synthase_type-III.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR11877; PTHR11877; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF00195; Chal_sti_synt_N; 1.
DR PIRSF; PIRSF000451; PKS_III; 1.
DR SUPFAM; SSF53901; SSF53901; 2.
DR PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE 3: Inferred from homology;
KW Acyltransferase; Cytoplasm; Flavonoid biosynthesis; Transferase.
FT CHAIN 1..389
FT /note="Chalcone synthase H2"
FT /id="PRO_0000452941"
FT ACT_SITE 164
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ SEQUENCE 389 AA; 42563 MW; E7346B45D4470ED1 CRC64;
MVTVEEVRKA QRAEGPATIL AIGTATPANC ILQSEYPDYY FRITNSEHKT ELKEKFKRMC
DKSMIRKRYM HLTEEILKEN PNLCAYEAPS LDARQDMVVV EVPKLGKEAA TKAIKEWGQP
KSKITHVVFC TTSGVDMPGA DYQLTKLLGL RPSVKRLMMY QQGCFAGGTV LRVAKDLAEN
NKGARVLVVC SEITAVTFRG PNDTHLDSLV GQALFGDGSA ALIIGADPIP EIEKPTFELV
SAAQTILPDS DGAIDGHLRE VGLTFHLLKD VPGLISKNIE KSLVEAFKPL GISDWNSLFW
IAHPGGPAIL DQVESKLALK PEKLRATRHV LGEYGNMSSA CVLFILDEMR RKCAEDGLKT
TGEGLEWGVL FGFGPGLTVE TVVLHSVGI