CHSJ_PETHY
ID CHSJ_PETHY Reviewed; 389 AA.
AC P22928;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 2.
DT 25-MAY-2022, entry version 91.
DE RecName: Full=Chalcone synthase J;
DE EC=2.3.1.74;
DE AltName: Full=Naringenin-chalcone synthase J;
GN Name=CHSJ;
OS Petunia hybrida (Petunia).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Petunioideae; Petunia.
OX NCBI_TaxID=4102;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Violet 30; TISSUE=Leaf;
RX PubMed=2806915; DOI=10.1016/0378-1119(89)90185-6;
RA Koes R.E., Spelt C.E., van den Elzen P.J.M., Mol J.N.M.;
RT "Cloning and molecular characterization of the chalcone synthase multigene
RT family of Petunia hybrida.";
RL Gene 81:245-257(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 71-389.
RC STRAIN=cv. White 137; TISSUE=Anther;
RA van Tunen A.J.;
RL Submitted (MAR-1989) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC which can under specific conditions spontaneously isomerize into
CC naringenin.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X14597; CAA32737.1; -; Genomic_DNA.
DR EMBL; X14599; CAA32739.1; -; mRNA.
DR PIR; D72821; SYPJCJ.
DR PIR; S18136; S18136.
DR AlphaFoldDB; P22928; -.
DR SMR; P22928; -.
DR UniPathway; UPA00154; -.
DR GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR InterPro; IPR011141; Polyketide_synthase_type-III.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR11877; PTHR11877; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF00195; Chal_sti_synt_N; 1.
DR PIRSF; PIRSF000451; PKS_III; 1.
DR SUPFAM; SSF53901; SSF53901; 2.
DR PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Flavonoid biosynthesis; Transferase.
FT CHAIN 1..389
FT /note="Chalcone synthase J"
FT /id="PRO_0000216034"
FT ACT_SITE 164
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
FT CONFLICT 75
FT /note="E -> V (in Ref. 2; CAA32739)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 389 AA; 42558 MW; F2B3CDD82E6FDE7D CRC64;
MVTVEEIRRA QRAEGPATIM AIGTATPSNC VDQSTYPDYY FRITNSEHKT ELKEKFQRMC
DKSMIKKRYM HLTEEILKEN PNICEYMAPS LDARQDIVVV EVPKLGKEAA QKAIKEWGQP
KSKITHLVFC TTSGVDMPGA DYQLTKLLGL RSSVKRLMMY QQGCFAGGTV LRLAKDLAEN
NKGARVLVVC SEITAVTFRG PNDTHLDSLV GQALFGDGAA AIIIGSDPLP GVERPLFELV
SASQTLLPDS EGAIDGHLRE VGLTFHLLKD VPGLISKNIQ KSLVEAFQPL GISDWNSIFW
IAHPGGPAIL DQVELKLGLK PEKLRATRHV LSEYGNMSSA CVLFILDEMR KASSKEGLGT
TGEGLEWGVL FGFGPGLTVE TVVLHSVST
