ID   CHSTA_DANRE             Reviewed;         365 AA.
AC   Q6AXM1;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Carbohydrate sulfotransferase 10;
DE            EC=2.8.2.-;
DE   AltName: Full=HNK-1 sulfotransferase;
DE            Short=HNK-1ST;
DE            Short=HNK1ST;
GN   Name=chst10; ORFNames=zgc:100964;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the transfer of sulfate to position 3 of terminal
CC       glucuronic acid of both protein- and lipid-linked oligosaccharides.
CC       Participates in biosynthesis of HNK-1 carbohydrate structure, a
CC       sulfated glucuronyl-lactosaminyl residue carried by many neural
CC       recognition molecules.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Single-
CC       pass type II membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the sulfotransferase 2 family. {ECO:0000305}.
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DR   EMBL; BC079482; AAH79482.1; -; mRNA.
DR   RefSeq; NP_001003779.1; NM_001003779.1.
DR   AlphaFoldDB; Q6AXM1; -.
DR   STRING; 7955.ENSDARP00000047210; -.
DR   PaxDb; Q6AXM1; -.
DR   Ensembl; ENSDART00000047211; ENSDARP00000047210; ENSDARG00000031632.
DR   GeneID; 445322; -.
DR   KEGG; dre:445322; -.
DR   CTD; 9486; -.
DR   ZFIN; ZDB-GENE-040808-40; chst10.
DR   eggNOG; KOG4651; Eukaryota.
DR   GeneTree; ENSGT00940000157128; -.
DR   HOGENOM; CLU_043398_2_0_1; -.
DR   InParanoid; Q6AXM1; -.
DR   OMA; NVCREDT; -.
DR   OrthoDB; 1330889at2759; -.
DR   PhylomeDB; Q6AXM1; -.
DR   TreeFam; TF325581; -.
DR   Reactome; R-DRE-975578; Reactions specific to the complex N-glycan synthesis pathway.
DR   PRO; PR:Q6AXM1; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 1.
DR   Bgee; ENSDARG00000031632; Expressed in pharyngeal gill and 35 other tissues.
DR   ExpressionAtlas; Q6AXM1; baseline and differential.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0008146; F:sulfotransferase activity; IBA:GO_Central.
DR   GO; GO:0016051; P:carbohydrate biosynthetic process; IEA:InterPro.
DR   GO; GO:0030166; P:proteoglycan biosynthetic process; IBA:GO_Central.
DR   InterPro; IPR018011; Carb_sulfotrans_8-10.
DR   InterPro; IPR005331; Sulfotransferase.
DR   PANTHER; PTHR12137; PTHR12137; 1.
DR   Pfam; PF03567; Sulfotransfer_2; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Glycoprotein; Golgi apparatus; Membrane;
KW   Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..365
FT                   /note="Carbohydrate sulfotransferase 10"
FT                   /id="PRO_0000189662"
FT   TOPO_DOM        1..6
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        7..27
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        28..356
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   BINDING         132..138
FT                   /ligand="3'-phosphoadenylyl sulfate"
FT                   /ligand_id="ChEBI:CHEBI:58339"
FT                   /evidence="ECO:0000250"
FT   BINDING         194..202
FT                   /ligand="3'-phosphoadenylyl sulfate"
FT                   /ligand_id="ChEBI:CHEBI:58339"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        99
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        104
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        325
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   365 AA;  42512 MW;  B0AB8AEE181D8CA1 CRC64;
     MRRHWLLVGA CGWVLLILMF VSKFINFSFR IPGDYAGRSE IFVWTLSSVT TKLPSVVWPE
     KGASQPYILS SVSPSVVEPI DWHLVNEKRL EQLSTVCSNS SIWNLTHTTV RKFVLDRIFV
     CDKHKILFCQ TPKVGNTQWK KVLIVLNGKF SKVEAIPENL VHDHERNGLP RLSSMTDTEI
     HQRLNSYFKF FIVRDPFERL ISAFKDKFVK NPRFEPWYKH DIAPAIVRKY RRSHHDDSES
     VGLRFEDFVR YLGDKTGRQH LDRQFGDHII HWLTYAELCA PCDISYNVVG HHETLELDAP
     YILKSAGIAG LVSYPSIPPG ITRYNRTKVE RYFSGISQRD IRRLYARYQG DFSLFDYPKP
     AFLLN