CHSTD_HUMAN
ID CHSTD_HUMAN Reviewed; 341 AA.
AC Q8NET6; Q3SYA3; Q3SYA5;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Carbohydrate sulfotransferase 13;
DE EC=2.8.2.5;
DE AltName: Full=Chondroitin 4-O-sulfotransferase 3;
DE AltName: Full=Chondroitin 4-sulfotransferase 3;
DE Short=C4ST-3;
DE Short=C4ST3;
GN Name=CHST13;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ENZYME ACTIVITY, AND TISSUE
RP SPECIFICITY.
RX PubMed=12080076; DOI=10.1074/jbc.m204907200;
RA Kang H.-G., Evers M.R., Xia G., Baenziger J.U., Schachner M.;
RT "Molecular cloning and characterization of chondroitin-4-O-
RT sulfotransferase-3. A novel member of the HNK-1 family of
RT sulfotransferases.";
RL J. Biol. Chem. 277:34766-34772(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), AND VARIANTS
RP VAL-271 AND GLN-317.
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- FUNCTION: Catalyzes the transfer of sulfate to position 4 of the N-
CC acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin
CC sulfate constitutes the predominant proteoglycan present in cartilage
CC and is distributed on the surfaces of many cells and extracellular
CC matrices. Transfers sulfate to the C4 hydroxyl of beta1,4-linked GalNAc
CC that is substituted with a beta-linked glucuronic acid at the C-3
CC hydroxyl. No activity toward dermatan.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=n 3'-phosphoadenylyl sulfate + chondroitin beta-D-glucuronate
CC = n adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate + n H(+);
CC Xref=Rhea:RHEA:16101, Rhea:RHEA-COMP:9827, Rhea:RHEA-COMP:9829,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57652, ChEBI:CHEBI:58339,
CC ChEBI:CHEBI:58343, ChEBI:CHEBI:58422; EC=2.8.2.5;
CC Evidence={ECO:0000269|PubMed:12080076};
CC -!- INTERACTION:
CC Q8NET6; Q5BVD1: TTMP; NbExp=3; IntAct=EBI-11599701, EBI-10243654;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Single-
CC pass type II membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8NET6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8NET6-2; Sequence=VSP_057175, VSP_057176;
CC -!- TISSUE SPECIFICITY: Highly expressed in adult liver. Expressed at lower
CC level in kidney, lymph nodes and fetal kidney.
CC {ECO:0000269|PubMed:12080076}.
CC -!- SIMILARITY: Belongs to the sulfotransferase 2 family. {ECO:0000305}.
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DR EMBL; AY120869; AAM55481.1; -; mRNA.
DR EMBL; BC103895; AAI03896.1; -; mRNA.
DR EMBL; BC103896; AAI03897.1; -; mRNA.
DR EMBL; BC103897; AAI03898.1; -; mRNA.
DR CCDS; CCDS3039.1; -. [Q8NET6-1]
DR RefSeq; NP_690849.1; NM_152889.2. [Q8NET6-1]
DR AlphaFoldDB; Q8NET6; -.
DR BioGRID; 127923; 5.
DR IntAct; Q8NET6; 2.
DR STRING; 9606.ENSP00000317404; -.
DR GlyGen; Q8NET6; 1 site.
DR iPTMnet; Q8NET6; -.
DR PhosphoSitePlus; Q8NET6; -.
DR BioMuta; CHST13; -.
DR EPD; Q8NET6; -.
DR jPOST; Q8NET6; -.
DR MassIVE; Q8NET6; -.
DR MaxQB; Q8NET6; -.
DR PaxDb; Q8NET6; -.
DR PeptideAtlas; Q8NET6; -.
DR PRIDE; Q8NET6; -.
DR ProteomicsDB; 61850; -.
DR ProteomicsDB; 73215; -. [Q8NET6-1]
DR Antibodypedia; 33086; 139 antibodies from 26 providers.
DR DNASU; 166012; -.
DR Ensembl; ENST00000319340.7; ENSP00000317404.2; ENSG00000180767.11. [Q8NET6-1]
DR GeneID; 166012; -.
DR KEGG; hsa:166012; -.
DR MANE-Select; ENST00000319340.7; ENSP00000317404.2; NM_152889.3; NP_690849.1.
DR UCSC; uc003eja.4; human. [Q8NET6-1]
DR CTD; 166012; -.
DR DisGeNET; 166012; -.
DR GeneCards; CHST13; -.
DR HGNC; HGNC:21755; CHST13.
DR HPA; ENSG00000180767; Group enriched (liver, testis).
DR MIM; 610124; gene.
DR neXtProt; NX_Q8NET6; -.
DR OpenTargets; ENSG00000180767; -.
DR PharmGKB; PA134976803; -.
DR VEuPathDB; HostDB:ENSG00000180767; -.
DR eggNOG; KOG4651; Eukaryota.
DR GeneTree; ENSGT00940000161154; -.
DR HOGENOM; CLU_043398_1_1_1; -.
DR InParanoid; Q8NET6; -.
DR OMA; RYFKHIS; -.
DR OrthoDB; 1330889at2759; -.
DR PhylomeDB; Q8NET6; -.
DR TreeFam; TF325581; -.
DR BioCyc; MetaCyc:HS11524-MON; -.
DR BRENDA; 2.8.2.5; 2681.
DR PathwayCommons; Q8NET6; -.
DR Reactome; R-HSA-2022870; Chondroitin sulfate biosynthesis.
DR SignaLink; Q8NET6; -.
DR BioGRID-ORCS; 166012; 14 hits in 1066 CRISPR screens.
DR ChiTaRS; CHST13; human.
DR GenomeRNAi; 166012; -.
DR Pharos; Q8NET6; Tbio.
DR PRO; PR:Q8NET6; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; Q8NET6; protein.
DR Bgee; ENSG00000180767; Expressed in right lobe of liver and 87 other tissues.
DR ExpressionAtlas; Q8NET6; baseline and differential.
DR Genevisible; Q8NET6; HS.
DR GO; GO:0000139; C:Golgi membrane; TAS:Reactome.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0047756; F:chondroitin 4-sulfotransferase activity; IDA:UniProtKB.
DR GO; GO:0001537; F:N-acetylgalactosamine 4-O-sulfotransferase activity; IDA:UniProtKB.
DR GO; GO:0008146; F:sulfotransferase activity; IBA:GO_Central.
DR GO; GO:0016051; P:carbohydrate biosynthetic process; IEA:InterPro.
DR GO; GO:0030206; P:chondroitin sulfate biosynthetic process; IDA:UniProtKB.
DR GO; GO:0030166; P:proteoglycan biosynthetic process; IBA:GO_Central.
DR InterPro; IPR018011; Carb_sulfotrans_8-10.
DR InterPro; IPR005331; Sulfotransferase.
DR PANTHER; PTHR12137; PTHR12137; 1.
DR Pfam; PF03567; Sulfotransfer_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Carbohydrate metabolism; Glycoprotein;
KW Golgi apparatus; Membrane; Phosphoprotein; Reference proteome;
KW Signal-anchor; Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..341
FT /note="Carbohydrate sulfotransferase 13"
FT /id="PRO_0000189671"
FT TOPO_DOM 1..9
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..30
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..341
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT MOTIF 132..134
FT /note="Cell attachment site"
FT /evidence="ECO:0000255"
FT BINDING 113..119
FT /ligand="3'-phosphoadenylyl sulfate"
FT /ligand_id="ChEBI:CHEBI:58339"
FT /evidence="ECO:0000250"
FT BINDING 174..182
FT /ligand="3'-phosphoadenylyl sulfate"
FT /ligand_id="ChEBI:CHEBI:58339"
FT /evidence="ECO:0000250"
FT MOD_RES 139
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT CARBOHYD 331
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 191..225
FT /note="PYSAAFQRRYGARIVQRLRPRALPDARARGHDVRF -> TRSATRVASATTS
FT WASSRRWRRTRPSCWAWRAHPT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_057175"
FT VAR_SEQ 226..341
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_057176"
FT VARIANT 146
FT /note="P -> S (in dbSNP:rs34311016)"
FT /id="VAR_053698"
FT VARIANT 271
FT /note="A -> V (in dbSNP:rs1056523)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_021477"
FT VARIANT 317
FT /note="R -> Q (in dbSNP:rs1056522)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_021478"
SQ SEQUENCE 341 AA; 38920 MW; A372134675D661F6 CRC64;
MGRRCCRRRV LAAACLGAAL LLLCAAPRSL RPAFGNRALG SSWLGGEKRS PLQKLYDLDQ
DPRSTLAKVH RQRRDLLNSA CSRHSRRQRL LQPEDLRHVL VDDAHGLLYC YVPKVACTNW
KRVLLALSGQ ARGDPRAISA QEAHAPGRLP SLADFSPAEI NRRLRAYLAF LFVREPFERL
ASAYRNKLAR PYSAAFQRRY GARIVQRLRP RALPDARARG HDVRFAEFLA YLLDPRTRRE
EPFNEHWERA HALCHPCRLR YDVVGKFETL AEDAAFVLGL AGASDLSFPG PPRPRGAAAS
RDLAARLFRD ISPFYQRRLF DLYKMDFLLF NYSAPSYLRL L
