ID   CHSTE_DANRE             Reviewed;         367 AA.
AC   Q805E5;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Carbohydrate sulfotransferase 14;
DE            EC=2.8.2.-;
DE   AltName: Full=Dermatan 4-sulfotransferase 1;
DE            Short=D4ST-1;
DE            Short=zD4ST-1;
GN   Name=chst14; Synonyms=d4st1;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Mizumoto S., Kobayashi N., Mikami T., Kitagawa H., Sugahara K.;
RT   "Molecular cloning and expression of zebrafish dermatan 4-
RT   sulfotransferase.";
RL   Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the transfer of sulfate to position 4 of the N-
CC       acetylgalactosamine (GalNAc) residue of dermatan sulfate.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Single-
CC       pass type II membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the sulfotransferase 2 family. {ECO:0000305}.
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DR   EMBL; AB089140; BAC55953.1; -; mRNA.
DR   RefSeq; NP_997994.1; NM_212829.1.
DR   AlphaFoldDB; Q805E5; -.
DR   STRING; 7955.ENSDARP00000063151; -.
DR   PaxDb; Q805E5; -.
DR   PRIDE; Q805E5; -.
DR   Ensembl; ENSDART00000063152; ENSDARP00000063151; ENSDARG00000043011.
DR   GeneID; 404732; -.
DR   KEGG; dre:404732; -.
DR   CTD; 113189; -.
DR   ZFIN; ZDB-GENE-040401-3; chst14.
DR   eggNOG; KOG4651; Eukaryota.
DR   GeneTree; ENSGT00940000162640; -.
DR   HOGENOM; CLU_043398_1_3_1; -.
DR   InParanoid; Q805E5; -.
DR   OMA; SQKNMPH; -.
DR   OrthoDB; 1330889at2759; -.
DR   PhylomeDB; Q805E5; -.
DR   TreeFam; TF325581; -.
DR   BRENDA; 2.8.2.35; 928.
DR   Reactome; R-DRE-2022923; Dermatan sulfate biosynthesis.
DR   PRO; PR:Q805E5; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 20.
DR   Bgee; ENSDARG00000043011; Expressed in swim bladder and 28 other tissues.
DR   ExpressionAtlas; Q805E5; baseline.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0001537; F:N-acetylgalactosamine 4-O-sulfotransferase activity; ISS:UniProtKB.
DR   GO; GO:0008146; F:sulfotransferase activity; IBA:GO_Central.
DR   GO; GO:0016051; P:carbohydrate biosynthetic process; IEA:InterPro.
DR   GO; GO:0050655; P:dermatan sulfate proteoglycan metabolic process; ISS:UniProtKB.
DR   InterPro; IPR018011; Carb_sulfotrans_8-10.
DR   InterPro; IPR005331; Sulfotransferase.
DR   PANTHER; PTHR12137; PTHR12137; 1.
DR   Pfam; PF03567; Sulfotransfer_2; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Glycoprotein; Golgi apparatus; Membrane;
KW   Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..367
FT                   /note="Carbohydrate sulfotransferase 14"
FT                   /id="PRO_0000189674"
FT   TOPO_DOM        1..34
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        35..55
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        56..367
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   BINDING         144..150
FT                   /ligand="3'-phosphoadenylyl sulfate"
FT                   /ligand_id="ChEBI:CHEBI:58339"
FT                   /evidence="ECO:0000250"
FT   BINDING         202..210
FT                   /ligand="3'-phosphoadenylyl sulfate"
FT                   /ligand_id="ChEBI:CHEBI:58339"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        99
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        359
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   367 AA;  42857 MW;  9FD8D6BB87705692 CRC64;
     MPPRKKEYGI KRASGSLVHF RAPVSATTIR RHSAVVPSVL TFAVIVASGG LLLMIEKGML
     NSVQTPPPRA NGRKVEYRLR SSSDTAADVE SQIVQEIRNR TIRSVCGQRN MPHSVWSLSP
     LQRKTLLQHI LVNDEHRFLY CYVPKVACSN WKRVLKVLSG ALANVDIKVK MDHRADLVFL
     SDLPPEEIRH RLRHYFKFMF VREPMARLLS AYRNKFGEIE AYQRKYGAEI IRRYRKGYAK
     DKKISGNDVT FTEFTRYLVD EDPERMNEHW MPIYNLCQPC AIEYDFIGSY ERLESDASYI
     LERVGAPQHV RFPERQTWYK PVTKETLHYY LCTVPQKFLK ELLPKYILDF SLFGYPLPNT
     TTEYCRH