ID   CHSY_ARAAL              Reviewed;         391 AA.
AC   Q9SEP4;
DT   25-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Chalcone synthase;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase;
GN   Name=CHS;
OS   Arabis alpina (Alpine rock-cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Arabideae; Arabis.
OX   NCBI_TaxID=50452;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=AlpAF;
RX   PubMed=11018155; DOI=10.1093/oxfordjournals.molbev.a026248;
RA   Koch M.A., Haubold B., Mitchell-Olds T.;
RT   "Comparative evolutionary analysis of chalcone synthase and alcohol
RT   dehydrogenase loci in Arabidopsis, Arabis, and related genera
RT   (Brassicaceae).";
RL   Mol. Biol. Evol. 17:1483-1498(2000).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; AF112083; AAF23558.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9SEP4; -.
DR   SMR; Q9SEP4; -.
DR   eggNOG; ENOG502S4A6; Eukaryota.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..391
FT                   /note="Chalcone synthase"
FT                   /id="PRO_0000215953"
FT   ACT_SITE        165
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   391 AA;  42658 MW;  103B92D7BDB20001 CRC64;
     MAPSLEEIRK AQRADGPAGI LGIGTANPPN HVLQAEYPDY YFRITNSDHM TDLKEKFKRM
     CDKSMIRKRH MHLTEEFLKE NPKMCAYMAP SLDTRQDIVV VEVPKLGKEA AVKAIKEWGQ
     PKSKITHVVF CTTSGVDMPG ADYQLTKLLG LRPSVKRLMM YQQGCFAGGT VLRLAKDLAE
     NNRGARVLVV CSEITAVTFR GPSDTHLDSL VGQALFSDGA AALIVGSDPD TSVGEKPIFE
     MVSAAQTILP DSDGAIDGHL REVGLTFHLL KDVPGLISKN IEKSLDEAFK PLGISDWNSL
     FWIAHPGGPA ILDQVEIKLG LKAEKMRATR HVLSEYGNMS SACVLFILDE MRKKSAKDGA
     ATTGEGLEWG VLFGFGPGLT VETVVLHSVP L