CHSY_CALCH
ID CHSY_CALCH Reviewed; 398 AA.
AC P48385;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-1998, sequence version 2.
DT 25-MAY-2022, entry version 81.
DE RecName: Full=Chalcone synthase;
DE EC=2.3.1.74;
DE AltName: Full=Naringenin-chalcone synthase;
GN Name=CHS;
OS Callistephus chinensis (China aster) (Callistemma chinense).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Asteroideae; Astereae;
OC Australasian lineages; Asterinae; Callistephus.
OX NCBI_TaxID=13379;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=L 01; TISSUE=Petal;
RA Henkel J., Wassenegger M., Sommer H., Forkmann G.;
RL Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC which can under specific conditions spontaneously isomerize into
CC naringenin.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000305}.
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DR EMBL; Z67988; CAA91930.1; -; mRNA.
DR AlphaFoldDB; P48385; -.
DR SMR; P48385; -.
DR UniPathway; UPA00154; -.
DR GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR InterPro; IPR011141; Polyketide_synthase_type-III.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR11877; PTHR11877; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF00195; Chal_sti_synt_N; 1.
DR PIRSF; PIRSF000451; PKS_III; 1.
DR SUPFAM; SSF53901; SSF53901; 2.
DR PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Flavonoid biosynthesis; Transferase.
FT CHAIN 1..398
FT /note="Chalcone synthase"
FT /id="PRO_0000215959"
FT ACT_SITE 167
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ SEQUENCE 398 AA; 43541 MW; 8B7D4E11B4FAFFC3 CRC64;
MASTIDIAAI REAQRRQGPA TILAIGTATP SNCVYQADYP DYYFRITKSE HMVDLKEKFK
RMCDKSMIRK RYMHLTEEYL KENPSLCEYM APSLDARQDV VVVEVPKLGK EAATKAIKEW
GQPKSKITHL IFCTTSGVDM PGADYQLTKL LGLRPSVKRF MMYQQGCFAG GTVLRLAKDL
AENNKGARVL VVCSEITAVT FRGPNDTHLD SLVGQALFGD GAAAVIVGAD PDLTTERPLF
EMISAAQTIL PDSEGAIDGH LREVGLTFHL LKDVPGLISK NIEKALTQAF SPLGITDWNS
IFWIAHPGGP AILDQVELKL GLKEEKMRAT RHVLSEYGNM SSACVLFIID EMRKKSAEDG
AATTGEGLDW GVLFGFGPGL TVETVVLHSL PTTMAIAT