ID   CHSY_CARAN              Reviewed;         395 AA.
AC   Q9SEP2;
DT   08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Chalcone synthase;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase;
GN   Name=CHS;
OS   Cardamine amara (Large bitter-cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Cardamineae; Cardamine.
OX   NCBI_TaxID=50461;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Cama;
RX   PubMed=11018155; DOI=10.1093/oxfordjournals.molbev.a026248;
RA   Koch M.A., Haubold B., Mitchell-Olds T.;
RT   "Comparative evolutionary analysis of chalcone synthase and alcohol
RT   dehydrogenase loci in Arabidopsis, Arabis, and related genera
RT   (Brassicaceae).";
RL   Mol. Biol. Evol. 17:1483-1498(2000).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; AF112085; AAF23560.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9SEP2; -.
DR   SMR; Q9SEP2; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   3: Inferred from homology;
KW   Acetylation; Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:P13114"
FT   CHAIN           2..395
FT                   /note="Chalcone synthase"
FT                   /id="PRO_0000215963"
FT   ACT_SITE        169
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
FT   MOD_RES         2
FT                   /note="N-acetylvaline"
FT                   /evidence="ECO:0000250|UniProtKB:P13114"
SQ   SEQUENCE   395 AA;  43204 MW;  1DADE8DF44DA7CA1 CRC64;
     MVMGDTPSLD EIRKAQRADG PAGILAIGTA NPANYVIQAE YPDYYFRITN SEHMTDLKEK
     FKRMCDKSTI RKRHMHVTEE FLKENPNMCA YMAPSLDARQ DIVVVEVPKL GKEAAVKAIK
     EWGQPKSKIT HLVFCTTSGV DMPGADYQLT KLLGLRPSVK RLMMYQQGCF AGGTVLRLAK
     DLAENNRGAR VLVVCSEITA VTFRGPSDTH LDSLVGQALF SDGAAALIVG SDPDTSVGEK
     PIFEMVSAAQ TILPDSDGAI DGHLREVGLT FHLLKDVPGL ISKNIEKSLD EAFKPLGISD
     WNSLFWIAHP GGPAILDQVE IKLGLKEEKM RATRHVMREY GNMSSACVLF ILDEMRKKSA
     KDGVATTGEG LEWGVLFGFG PGLTVETVVL HSVPL