ID   CHSY_CATRO              Reviewed;         389 AA.
AC   Q9ZRS4;
DT   08-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Chalcone synthase;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase;
GN   Name=CHS;
OS   Catharanthus roseus (Madagascar periwinkle) (Vinca rosea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Gentianales; Apocynaceae; Rauvolfioideae; Vinceae;
OC   Catharanthinae; Catharanthus.
OX   NCBI_TaxID=4058;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=10476065; DOI=10.1046/j.1365-313x.1999.00524.x;
RA   Kaltenbach M., Schroeder G., Schmelzer E., Lutz V., Schroeder J.;
RT   "Flavonoid hydroxylase from Catharanthus roseus: cDNA, heterologous
RT   expression, enzyme properties and cell-type specific expression in
RT   plants.";
RL   Plant J. 19:183-193(1999).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; AJ131813; CAA10511.1; -; mRNA.
DR   AlphaFoldDB; Q9ZRS4; -.
DR   SMR; Q9ZRS4; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..389
FT                   /note="Chalcone synthase"
FT                   /id="PRO_0000215965"
FT   ACT_SITE        164
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   389 AA;  42492 MW;  B350742DE4A19186 CRC64;
     MVNVEEIRNA QRAQGPATVL AIGTSTPSNC VDQSTYPDYY FRITNSEHKT ELKEKFKRMC
     EKSMIKKRYM HLTEEILQEN PNICAYMAPS LDARQNIVVV EVPKLGKEAA QKAIKEWGQS
     KSKITHLVFC TTSGVDMPGA DYQLTKLLGL RSSVKRLMMY QQGCFAGGTV LRLAKDLAEN
     NKGARVLVVC SEITAVTFRG PSESHLDSLV GQALFGDGAA AIIVGSDPIP EIERPLFELV
     SAAQTLLPDS HGAIDGHLRE VGLTFHLLKD VPGLISKNIG KALDEAFQPL GISDWNSIFW
     IAHPGGPAIL DQVEEKLGLK PEKLRATRHV LSEYGNMSSA CVLFILDEMR KASARDGLST
     TGEGLEWGVL FGFGPGLTVE TVVLHSVNV