ID   CHSY_CHRAE              Reviewed;         396 AA.
AC   O04220;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Chalcone synthase;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase;
GN   Name=CHS;
OS   Chrysosplenium americanum (American golden saxifrage).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Saxifragales; Saxifragaceae; Chrysosplenium.
OX   NCBI_TaxID=36749;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Gauthier A., Gulick P.J., Ibrahim R.K.;
RT   "A cDNA encoding chalcone synthase from Chrysosplenium americanum.";
RL   Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; U96661; AAB54075.1; -; mRNA.
DR   AlphaFoldDB; O04220; -.
DR   SMR; O04220; -.
DR   PRIDE; O04220; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..396
FT                   /note="Chalcone synthase"
FT                   /id="PRO_0000215966"
FT   ACT_SITE        167
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   396 AA;  43415 MW;  A9EEB6CC8FD7722C CRC64;
     MSSAALMEEI RNAQRAEGPA TILAIGTATP ANCVIQADYP DFYFRITNSE HKTELKEKFQ
     RMCDKSMIKK RYMHLTEDLL KENPKMCEYM APSLDARQDM VVVEIRKLGK EAAVKAIKEW
     GQPKSKITHL VFCTTSGVDM PGADYQLTKL LGLRPSVKRL MMYQQGCFPG GTVLRLAKDL
     AENNRGARVL VVCSEITAVT FRGPSDTHLD SLVGQALFGD GAAALIVGAD PDTAIERPLF
     ELVSAAQTIL PDSDGAIDGH LREVGLTFHL LKDVPGLISK NIEKSLIEAF KPIGINDWNS
     IFWIAHPGGP AILDQVEHKL APGRRANSRA TRHILSEYGN MSSACVLFIL DEMRKKSARR
     REATTGDGLE WGVLFGFGPG LTVETVVLHS VPAITA