ID   CHSY_DIACA              Reviewed;         391 AA.
AC   P48389;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Chalcone synthase;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase;
GN   Name=CHS;
OS   Dianthus caryophyllus (Carnation) (Clove pink).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Caryophyllaceae; Caryophylleae; Dianthus.
OX   NCBI_TaxID=3570;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Tanga; TISSUE=Petal;
RA   Henkel J., Sommer H., Forkmann G.;
RL   Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; Z67982; CAA91923.1; -; mRNA.
DR   PIR; T10713; T10713.
DR   AlphaFoldDB; P48389; -.
DR   SMR; P48389; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..391
FT                   /note="Chalcone synthase"
FT                   /id="PRO_0000215973"
FT   ACT_SITE        164
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   391 AA;  42795 MW;  30C8E2FF60E1E63F CRC64;
     MASIEEIRQA PRADGPATIL AIGTATPPNA IYQADYPDYY FRVTKSEHMT ELKEKFRRMC
     DKSMIKKRYM YLTEEILKEN PNLCEYMGSS LDTRQDMVVS EVPRLGKEAA VKAIKEWGQP
     KSKITHVIMC TTSGVDMPGA DYQLTKLLGL RPSVRRFMLY QQGCFAGGTV LRLAKDLAEN
     NKDARVLVVC SEITAICFRG PTEAALDSMV GQALFGDGAG ALIVGSDPDL SIERPLFQMA
     WAGQTLLPDS DGAIDGHLRE VGLTFHLLKD VPGIISKNIT NALEDAFSPI GVSDWNNLFW
     IAHPGGPAIL DQVEAKLGLK EEKLAATRNV LSDFGNMSSA CVLFILDEMR KKSLRDGATT
     TGEGLDWGVL FGFGPSLTVE TVVLHSVPLN C