ID   CHSY_DIAMO              Reviewed;         391 AA.
AC   Q9LKP7;
DT   08-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Chalcone synthase;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase;
GN   Name=CHS;
OS   Dianthus monspessulanus.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Caryophyllaceae; Caryophylleae; Dianthus.
OX   NCBI_TaxID=129769;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Yagishita Y., Ohya T., Kaminishi A., Nomura K., Kita N.;
RT   "Molecular cloning of chalcone synthase cDNA from Dianthus
RT   monspessulanus.";
RL   Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; AF267173; AAF81743.1; -; mRNA.
DR   AlphaFoldDB; Q9LKP7; -.
DR   SMR; Q9LKP7; -.
DR   PRIDE; Q9LKP7; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..391
FT                   /note="Chalcone synthase"
FT                   /id="PRO_0000215974"
FT   ACT_SITE        164
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   391 AA;  42766 MW;  7C132D24E66C6C5D CRC64;
     MASIEEIRQA QRADGPATIL AIGTATPPNA IYQADYPDYY FRVTKSEHMT ELKEKFRRMC
     DKSMIKKRYM YLTEEILKEN PNLCEYMGSS LDTRQDMVVS EVPRLGKEAA VKAIKEWGQP
     KSKITHVIMC TTSGVDMPGA DYQLTKLLGL RPSVRRFMLY QQGCFAGGTV LRLAKDLAEN
     NKGARVLVVC SEITAICFRG PTEAALDSMV GQALFGDGAG ALIVGSDPDL SIERPLFQMA
     WAGQTLLPDS EGAIDGHLRE VGLTFHLLKD VPGIISKNIT NALEEAFSPI GVSDWNNLFW
     IAHPGGPAIL DQVEAKLGLK EEKLAATRNV LSDFGNMSSA CVLFILDEMR KKSLRDGATT
     TGEGLDWGVL FGFGPGLTVE TVVLHSVPLN C