CHSY_EQUAR
ID CHSY_EQUAR Reviewed; 405 AA.
AC Q9MBB1;
DT 08-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 72.
DE RecName: Full=Chalcone synthase;
DE EC=2.3.1.74;
DE AltName: Full=Naringenin-chalcone synthase;
GN Name=CHS;
OS Equisetum arvense (Field horsetail) (Common horsetail).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Polypodiopsida; Equisetidae; Equisetales; Equisetaceae; Equisetum.
OX NCBI_TaxID=3258;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Yamazaki Y., Sankawa U.;
RL Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC which can under specific conditions spontaneously isomerize into
CC naringenin.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000305}.
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DR EMBL; AB030004; BAA89501.1; -; mRNA.
DR PDB; 6DX9; X-ray; 1.50 A; A/B=1-405.
DR PDBsum; 6DX9; -.
DR AlphaFoldDB; Q9MBB1; -.
DR SMR; Q9MBB1; -.
DR PRIDE; Q9MBB1; -.
DR UniPathway; UPA00154; -.
DR GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR InterPro; IPR011141; Polyketide_synthase_type-III.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR11877; PTHR11877; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF00195; Chal_sti_synt_N; 1.
DR PIRSF; PIRSF000451; PKS_III; 1.
DR SUPFAM; SSF53901; SSF53901; 2.
DR PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acyltransferase; Flavonoid biosynthesis; Transferase.
FT CHAIN 1..405
FT /note="Chalcone synthase"
FT /id="PRO_0000215975"
FT ACT_SITE 170
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
FT STRAND 24..31
FT /evidence="ECO:0007829|PDB:6DX9"
FT STRAND 34..38
FT /evidence="ECO:0007829|PDB:6DX9"
FT HELIX 39..41
FT /evidence="ECO:0007829|PDB:6DX9"
FT HELIX 42..49
FT /evidence="ECO:0007829|PDB:6DX9"
FT HELIX 56..67
FT /evidence="ECO:0007829|PDB:6DX9"
FT STRAND 72..77
FT /evidence="ECO:0007829|PDB:6DX9"
FT HELIX 80..85
FT /evidence="ECO:0007829|PDB:6DX9"
FT HELIX 87..89
FT /evidence="ECO:0007829|PDB:6DX9"
FT STRAND 91..93
FT /evidence="ECO:0007829|PDB:6DX9"
FT HELIX 97..123
FT /evidence="ECO:0007829|PDB:6DX9"
FT HELIX 127..129
FT /evidence="ECO:0007829|PDB:6DX9"
FT STRAND 132..139
FT /evidence="ECO:0007829|PDB:6DX9"
FT HELIX 146..154
FT /evidence="ECO:0007829|PDB:6DX9"
FT STRAND 161..167
FT /evidence="ECO:0007829|PDB:6DX9"
FT HELIX 172..185
FT /evidence="ECO:0007829|PDB:6DX9"
FT STRAND 191..198
FT /evidence="ECO:0007829|PDB:6DX9"
FT TURN 201..203
FT /evidence="ECO:0007829|PDB:6DX9"
FT HELIX 212..220
FT /evidence="ECO:0007829|PDB:6DX9"
FT STRAND 224..233
FT /evidence="ECO:0007829|PDB:6DX9"
FT STRAND 242..251
FT /evidence="ECO:0007829|PDB:6DX9"
FT STRAND 258..264
FT /evidence="ECO:0007829|PDB:6DX9"
FT STRAND 267..271
FT /evidence="ECO:0007829|PDB:6DX9"
FT HELIX 276..295
FT /evidence="ECO:0007829|PDB:6DX9"
FT HELIX 297..299
FT /evidence="ECO:0007829|PDB:6DX9"
FT HELIX 302..304
FT /evidence="ECO:0007829|PDB:6DX9"
FT HELIX 309..311
FT /evidence="ECO:0007829|PDB:6DX9"
FT STRAND 312..316
FT /evidence="ECO:0007829|PDB:6DX9"
FT HELIX 321..331
FT /evidence="ECO:0007829|PDB:6DX9"
FT TURN 335..338
FT /evidence="ECO:0007829|PDB:6DX9"
FT HELIX 339..348
FT /evidence="ECO:0007829|PDB:6DX9"
FT HELIX 352..354
FT /evidence="ECO:0007829|PDB:6DX9"
FT HELIX 355..369
FT /evidence="ECO:0007829|PDB:6DX9"
FT TURN 375..378
FT /evidence="ECO:0007829|PDB:6DX9"
FT STRAND 380..388
FT /evidence="ECO:0007829|PDB:6DX9"
FT TURN 389..391
FT /evidence="ECO:0007829|PDB:6DX9"
FT STRAND 392..400
FT /evidence="ECO:0007829|PDB:6DX9"
SQ SEQUENCE 405 AA; 44216 MW; F20F5701381813A5 CRC64;
MTVLEESADA SSRRLAQRAN GPATVLAIGT ANPANVFEQS SYPDFYFDIT NSQHMTELKL
KFSRMCQKSG IKKRYMHLNS EILKANPSLC AYWEKSLDVR QDIAVVEVPK LGKEASLKAI
KEWGQPKSKI THLVFCTTSG VDMPGADWAL TKLLGLRPSV KRLMMYQQGC FAGGTVLRVA
KDVAENNKGA RVLVVCSEIT CVTFRGPSET HLDSLVGQAL FGDGAAAVIL GSDPLPEENP
CFELHWSGSN ILPDSDGAID GHLREVGLTF HLMKDVPGII SKNIGKVLND AFRSAFDESG
NAEDRPASVN DIFWIAHPGG PAILDQVEEK MKLAPEKMRA TRDVLSEYGN MSSACVLFIM
DHMRRMSAQN KLQTTGEGLD WGVLLGFGPG LTVETVLLKS IRLAC
