ID   CHSY_MALDO              Reviewed;         232 AA.
AC   P30078;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Chalcone synthase;
DE            EC=2.3.1.74;
DE   AltName: Full=Naregenin-chalcone synthase;
DE   Flags: Fragment;
GN   Name=CHS;
OS   Malus domestica (Apple) (Pyrus malus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Maleae; Malus.
OX   NCBI_TaxID=3750;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Leaf;
RA   Podivinsky E., Bradley J.M., Davis K.M.;
RL   Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X68977; CAA48773.1; -; mRNA.
DR   PIR; S29556; S29556.
DR   AlphaFoldDB; P30078; -.
DR   SMR; P30078; -.
DR   STRING; 3750.XP_008380609.1; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           <1..232
FT                   /note="Chalcone synthase"
FT                   /id="PRO_0000216015"
FT   ACT_SITE        7
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
FT   NON_TER         1
SQ   SEQUENCE   232 AA;  24616 MW;  12D6113C80A9E86B CRC64;
     MMYQQGCFAG GTVLRLAKDL AENNKGARVL VVCSEITAVT FRGPSDTHLD SLVGQALFGD
     GAAAVIIGAD PVPEVEKPLF ELVSAAQTVL PDSDGAIDGH LREVGLTFHL LKDVPGLISK
     NIEKSLNEAL KPIGISDWNS LFWIAHPGGP AILDQVEAKL ALKPEKLEAT RQVLSDYGNM
     SSACVLFILD EVRRKSAEKG LETTGEGLEW GVLFGFGPGL TVETVVLHSV AA