ID   CHSY_PERAE              Reviewed;         392 AA.
AC   Q9ZU06;
DT   08-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Chalcone synthase;
DE            EC=2.3.1.74;
DE   AltName: Full=Naregenin-chalcone synthase;
GN   Name=CHS;
OS   Persea americana (Avocado).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Magnoliidae; Laurales; Lauraceae; Persea.
OX   NCBI_TaxID=3435;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Ardi R., Kobiler I., Jacoby B., Keen N.T., Prusky D.;
RT   "Involvement of epicatechin biosynthesis in the activation of the mechanism
RT   of resistance of avocado fruits to Colletotrichum gloeosporioides.";
RL   Physiol. Mol. Plant Pathol. 53:269-286(1998).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; AD001672; AAC98143.1; -; mRNA.
DR   AlphaFoldDB; Q9ZU06; -.
DR   SMR; Q9ZU06; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..392
FT                   /note="Chalcone synthase"
FT                   /id="PRO_0000216026"
FT   ACT_SITE        165
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   392 AA;  42480 MW;  B08D286FB80BB42E CRC64;
     MVNVEAIRKV QRAEGPATIM AIGTSTPPNA VDQSEYPDYY YFGSPTASTR PSSRRSFKRM
     CEKSMIKKRY MYLTETYWKR IQMFVPTWLL PLKARQDMVV VEVPKLGKEV QPKAIKGMGQ
     PKSKINPLVF CTTSGVDMPG ADYQLTKVFG LPPSVKRLMM YQQGCFAGGT VLRLAKDLAE
     NNKGARVLVV CSEITAVTFR GPSDTHLDSL VGQALFGDGA AALIVGADPV PGVENPMFEL
     VSAGQTILPD SDGAIDGHLR EVGLTFHLLK VVPGLISKNI EKSLVEAFEP LGISDWNSLF
     WIAHPGGPAI LDPGGDQTRP EARESCGNQA CFSVSMATCQ VFVCSSFSTR CEGSPKEEGL
     KTTGEGIEWG VLFGFGPGLT VETVVLHSLP TH