ID   CHSY_PERFR              Reviewed;         391 AA.
AC   O04111;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Chalcone synthase;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase;
GN   Name=CHS;
OS   Perilla frutescens (Beefsteak mint) (Perilla ocymoides).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Lamiales; Lamiaceae; Nepetoideae; Elsholtzieae; Perilla.
OX   NCBI_TaxID=48386;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=9426610; DOI=10.1023/a:1005959203396;
RA   Gong Z., Yamazaki M., Sugiyama M., Tanaka Y., Saito K.;
RT   "Cloning and molecular analysis of structural genes involved in anthocyanin
RT   biosynthesis and expressed in a forma-specific manner in Perilla
RT   frutescens.";
RL   Plant Mol. Biol. 35:915-927(1997).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; AB002815; BAA19656.1; -; mRNA.
DR   AlphaFoldDB; O04111; -.
DR   SMR; O04111; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..391
FT                   /note="Chalcone synthase"
FT                   /id="PRO_0000216027"
FT   ACT_SITE        164
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   391 AA;  42686 MW;  AE071BE50845242E CRC64;
     MVTVEDIRRA QRAEGPATVM AIGTATPENC VDQSTYPDYY FRITNSEHRT DLKEKFKRMC
     EKSMIRKRYM HLTEEFLKEN PNMTAYMAPS LDARQDIVVV EVPKLGKEAA QKAIKEWGQP
     KSKITHLVFC TTSGVDMPGA DYQLTKLLGL RPSVKRFMMY QQGCFAGGTV LRMAKDLAEN
     NAGARVLVVC SEITAVTFRG PSESHLDSLV GQALFGDGAA AVIVGSDPVV GVERPLFQLV
     SAAQTILPDS DGAIDGHLRE VGLTFHLLKD VPGLISKNIE KSLKEAFGPL GISDWNSVFW
     IAHPGGPAIL DQVEAKLGLK PEKLRSTRHV LGEYGNMSSA CVLFILDEMR KSSAKEGMSS
     TGEGLDWGVL FGFGPGLTVE TVVLHSVPIN N