ID   CHSY_PETCR              Reviewed;         398 AA.
AC   P16107;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Chalcone synthase;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase;
GN   Name=CHS;
OS   Petroselinum crispum (Parsley) (Petroselinum hortense).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Apiales; Apiaceae; Apioideae; apioid superclade;
OC   Apieae; Petroselinum.
OX   NCBI_TaxID=4043;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=16453477; DOI=10.1002/j.1460-2075.1983.tb01661.x;
RA   Reimold U., Kroeger M., Kreuzaler F., Hahlbrock K.;
RT   "Coding and 3' non-coding nucleotide sequence of chalcone synthase mRNA and
RT   assignment of amino acid sequence of the enzyme.";
RL   EMBO J. 2:1801-1805(1983).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; V01538; CAA24779.1; -; Genomic_DNA.
DR   PIR; S42523; S42523.
DR   AlphaFoldDB; P16107; -.
DR   SMR; P16107; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..398
FT                   /note="Chalcone synthase"
FT                   /id="PRO_0000216028"
FT   ACT_SITE        169
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   398 AA;  43735 MW;  E8B19149AD3DAB1E CRC64;
     MANHHNAEIE EIRNRQRAQG PANILAIGTA TPSNCVYQAD YPDYYFRITN SEHMTDLKLK
     FKRMCEKSMI RKRYMHITEE YLKENPNVCA YEAPSLDARQ DLVVVEVPRL GKEAASKAIK
     EWGQPKSKIT HLIFCTTSGV DMPGADYQLT KLLGLRPSVK RFMMYQQGCF AGGTVLRLAK
     DLAENNAGAR VLVVCSEITA VTFRGPSDSH LDSLVGQALF GDGAAAVILG SDPDLSVERP
     LFQLISAAQT ILPDSDGAID GHLREVGLTF HLLKDVPGLI SKNIEKSLKE AFGPIGISDW
     NSLFWIAHPG GPAILDQVEL KLGLKEEKMR ATRQVLSDYG NMSSACVLFI LDEMRKKSIE
     EGKATTGEGL DWGVLFGFGP GLTVETVVLH SVPATFTH