ID   CHSY_PINST              Reviewed;         395 AA.
AC   O65872;
DT   08-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Chalcone synthase;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase;
GN   Name=CHS;
OS   Pinus strobus (Eastern white pine).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Pinus;
OC   Pinus subgen. Strobus.
OX   NCBI_TaxID=3348;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Schroeder J.;
RT   "Plant polyketide synthases: a chalcone synthase-type enzyme that performs
RT   with methylmalonyl-CoA a specific condensation reaction in the biosynthesis
RT   of C-methylated chalcones.";
RL   Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; AJ004800; CAA06077.1; -; mRNA.
DR   AlphaFoldDB; O65872; -.
DR   SMR; O65872; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..395
FT                   /note="Chalcone synthase"
FT                   /id="PRO_0000216041"
FT   ACT_SITE        169
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   395 AA;  43207 MW;  45E0A370189A4626 CRC64;
     MPGGMMADLE AFRKAQRADG PATILAIGTA TPPNAVDQST YPDYYFKITN SEHMTELKEK
     FRRMCDKSGI KKRYMYLTEE ILNENPSVCA YMAPSLDARQ DMVVVEVPRL GKEAAAKAIK
     EWGQPKSKIT HVIFCTTSGV DMPGADYQMT KLLGLRPSVK RVMMYQQGCF AGGTVLRVAK
     DLAENNRGAR VLVVCSEITA VTFRGPSDTH LDSMVGQALF GDGARALIVG ADPVPEVEKP
     CFEMLWTAQT ILPDSDGAID GHLREVGLTF HLLKDVPGLI SKNIEKSLVE AFQQFGISDW
     NQLFWIAHPG GPAILDQVEA KLNLDPKKLR ATRQVLSEYG NMSSACVHFI LDEMRKSSQQ
     NGCSTTGEGL DVGVLFGFGP GLTVETVVLK SVPLQ