CHSY_PUEML
ID CHSY_PUEML Reviewed; 389 AA.
AC P23569;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1991, sequence version 1.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Chalcone synthase;
DE EC=2.3.1.74;
DE AltName: Full=Naringenin-chalcone synthase;
GN Name=CHS;
OS Pueraria montana var. lobata (Kudzu vine) (Pueraria lobata).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Pueraria.
OX NCBI_TaxID=3893;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RX PubMed=1777944; DOI=10.1248/cpb.39.1911;
RA Nakajima O., Akiyama T., Hakamatsuka T., Shibuya M., Noguchi H.,
RA Ebizuka Y., Sankawa U.;
RT "Isolation, sequence and bacterial expression of a cDNA for chalcone
RT synthase from the cultured cells of Pueraria lobata.";
RL Chem. Pharm. Bull. 39:1911-1913(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-59.
RX PubMed=8820915; DOI=10.1248/bpb.19.71;
RA Nakajima O., Shibuya M., Hakamatsuka T., Noguchi H., Ebizuka Y.,
RA Sankawa U.;
RT "cDNA and genomic DNA clonings of chalcone synthase from Pueraria lobata.";
RL Biol. Pharm. Bull. 19:71-76(1996).
CC -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC which can under specific conditions spontaneously isomerize into
CC naringenin.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000305}.
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DR EMBL; D10223; BAA01075.1; -; mRNA.
DR EMBL; D63855; BAA09918.1; -; Genomic_DNA.
DR PIR; JQ0911; SYFJCP.
DR AlphaFoldDB; P23569; -.
DR SMR; P23569; -.
DR UniPathway; UPA00154; -.
DR GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR InterPro; IPR011141; Polyketide_synthase_type-III.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR11877; PTHR11877; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF00195; Chal_sti_synt_N; 1.
DR PIRSF; PIRSF000451; PKS_III; 1.
DR SUPFAM; SSF53901; SSF53901; 2.
DR PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Flavonoid biosynthesis; Transferase.
FT CHAIN 1..389
FT /note="Chalcone synthase"
FT /id="PRO_0000216043"
FT ACT_SITE 164
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ SEQUENCE 389 AA; 42832 MW; 438F6DC77A37F5DE CRC64;
MVSVAEIRQA QRAEGPATIL AIGTANPPNC VDQSTYPDYY FRITNSEHMT ELKEKFQRMC
DKSMIKKRYM YLTEEILKEN PNMCAYMAPS LDARQDMVVV EVPKLGKEAA TKAIKEWGQP
KSKITHLIFC TTSGVDMPGA DYQLTKQLGL RPYVKRYMMY QQGCFAGGTV LRLAKDLAEN
NKGARVLVVC SEITAVTFRG PSDTHLDSLV GQALFGDGAA AVIVGSDPIP QVEKPLYELV
WTAQTIAPDS EGAIDGHLRE VGLTFHLLKD VPGIVSKNID KALFEAFNPL NISDYNSIFW
IAHPGGPAIL DQVEQKLGLK PEKMKATRDV LSDYGNMSSA CVLFILDEMR RKSAENGLKT
TGEGLEWGVL FGFGPGLTIE TVVLRSVAI
