ID   CHSY_RAPSA              Reviewed;         394 AA.
AC   O22652;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Chalcone synthase;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase;
GN   Name=CHS;
OS   Raphanus sativus (Radish) (Raphanus raphanistrum var. sativus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Raphanus.
OX   NCBI_TaxID=3726;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Kwon S.I., An C.-S.;
RL   Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; AF031922; AAB87072.1; -; mRNA.
DR   AlphaFoldDB; O22652; -.
DR   SMR; O22652; -.
DR   UniPathway; UPA00154; -.
DR   Proteomes; UP000504610; Unplaced.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Reference proteome; Transferase.
FT   CHAIN           1..394
FT                   /note="Chalcone synthase"
FT                   /id="PRO_0000216044"
FT   ACT_SITE        168
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   394 AA;  42892 MW;  5324498DFCB13DB0 CRC64;
     MVGTTSSLDE IRKAQRADGP AGILAIGTAN PANHVLQAEY PDYYFRITNS EHMTDLKEKF
     KRMCDKSTIR KRHMHLTEEF LKENPNMCAY MAPSLDARQD IVVVEVPKLG KEAAVKAIKE
     WGQPKSKITH VVFCTTSGVD MPGADYQLTK LLGLRPSVKR LMMYQQGCFA GGTVLRLAKD
     LAENNRGARV LVVCSEITAV TFRGPSDTHL DSLVGQALFS DGAAALIVGS DPDVSAGEKP
     IFEMVSAAQT ILPDSDGAID GHLREVGITF HLLKDVPGLI SKNIEKSLDE AFKPLGISDW
     NSLFWIAHPG GPAILDDVEK KLGLKAEKMR ATRHVLSEYG NMSSACVLFI LDEMRRKSLD
     DGVATTGEGL EWGVLFGFGP GLTVETVVLH SVPV