ID   CHS_CANSA               Reviewed;         389 AA.
AC   Q8RVK9;
DT   06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Naringenin-chalcone synthase;
DE            EC=2.3.1.74;
GN   Name=CHS; Synonyms=CAN1069;
OS   Cannabis sativa (Hemp) (Marijuana).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Rosales; Cannabaceae; Cannabis.
OX   NCBI_TaxID=3483;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, AND
RP   TISSUE SPECIFICITY.
RC   STRAIN=cv. Four-way; TISSUE=Leaf;
RX   PubMed=15120113; DOI=10.1016/j.plaphy.2004.02.011;
RA   Raharjo T.J., Chang W.T., Verberne M.C., Peltenburg-Looman A.M.,
RA   Linthorst H.J., Verpoorte R.;
RT   "Cloning and over-expression of a cDNA encoding a polyketide synthase from
RT   Cannabis sativa.";
RL   Plant Physiol. Biochem. 42:291-297(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, AND
RP   TISSUE SPECIFICITY.
RX   PubMed=19581347; DOI=10.1093/jxb/erp210;
RA   Marks M.D., Tian L., Wenger J.P., Omburo S.N., Soto-Fuentes W., He J.,
RA   Gang D.R., Weiblen G.D., Dixon R.A.;
RT   "Identification of candidate genes affecting Delta9-tetrahydrocannabinol
RT   biosynthesis in Cannabis sativa.";
RL   J. Exp. Bot. 60:3715-3726(2009).
CC   -!- FUNCTION: Chalcone synthase that can also use isovaleryl-CoA,
CC       isobutyryl-CoA or hexanoyl-CoA as substrates, but that is unable
CC       produce olivetol or olivetolic acid.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023,
CC         ECO:0000269|PubMed:15120113, ECO:0000269|PubMed:19581347};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in glandular trichomes. Detected at low
CC       levels in female flowers, stems, seeds, leaves and roots.
CC       {ECO:0000269|PubMed:15120113, ECO:0000269|PubMed:19581347}.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; AY082343; AAL92879.1; -; mRNA.
DR   AlphaFoldDB; Q8RVK9; -.
DR   SMR; Q8RVK9; -.
DR   EnsemblPlants; evm.model.07.160; cds.evm.model.07.160; evm.TU.07.160.
DR   Gramene; evm.model.07.160; cds.evm.model.07.160; evm.TU.07.160.
DR   OMA; YMHINEE; -.
DR   BioCyc; MetaCyc:MON-12039; -.
DR   GO; GO:0034081; C:polyketide synthase complex; IDA:UniProtKB.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IDA:UniProtKB.
DR   GO; GO:1901696; P:cannabinoid biosynthetic process; NAS:UniProtKB.
DR   GO; GO:0009715; P:chalcone biosynthetic process; IDA:UniProtKB.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   1: Evidence at protein level;
KW   Acyltransferase; Cytoplasm; Transferase.
FT   CHAIN           1..389
FT                   /note="Naringenin-chalcone synthase"
FT                   /id="PRO_0000421153"
FT   ACT_SITE        164
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   389 AA;  42720 MW;  51C5C0C87EFF6279 CRC64;
     MVTVEEFRKA QRAEGPATIM AIGTATPANC VLQSEYPDYY FRITNSEHKT ELKEKFKRMC
     DKSMIRKRYM HLTEEILKEN PNLCAYEAPS LDARQDMVVV EVPKLGKEAA TKAIKEWGQP
     KSKITHLVFC TTSGVDMPGA DYQLTKLLGL RPSVKRLMMY QQGCFAGGTV LRLAKDLAEN
     NKGARVLVVC SEITAVTFRG PNDTHLDSLV GQALFGDGSA ALIVGSDPIP EVEKPIFELV
     SAAQTILPDS DGAIDGHLRE VGLTFHLLKD VPGLISKNIE KSLNEAFKPL GISDWNSLFW
     IAHPGGPAIL DQVESKLALK TEKLRATRHV LSEYGNMSSA CVLFILDEMR RKCVEDGLNT
     TGEGLEWGVL FGFGPGLTVE TVVLHSVAI