CHUR_DANRE
ID CHUR_DANRE Reviewed; 112 AA.
AC Q5U3N7; Q5SNR5;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Protein Churchill;
GN Name=churc1; Synonyms=chch; ORFNames=si:dkey-12h9.10;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Larva;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION.
RX PubMed=17980025; DOI=10.1186/1471-213x-7-120;
RA Londin E.R., Mentzer L., Sirotkin H.I.;
RT "Churchill regulates cell movement and mesoderm specification by repressing
RT Nodal signaling.";
RL BMC Dev. Biol. 7:120-120(2007).
RN [4]
RP INDUCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=17521969; DOI=10.1016/j.modgep.2007.04.002;
RA Londin E.R., Mentzer L., Gates K.P., Sirotkin H.I.;
RT "Expression and regulation of the zinc finger transcription factor
RT Churchill during zebrafish development.";
RL Gene Expr. Patterns 7:645-650(2007).
CC -!- FUNCTION: Transcriptional activator that mediates FGF signaling during
CC neural development (By similarity). Plays a role in the regulation of
CC cell movement (PubMed:17980025). Does not bind DNA by itself (By
CC similarity). {ECO:0000250|UniProtKB:Q8WUH1,
CC ECO:0000250|UniProtKB:Q9DFZ3, ECO:0000269|PubMed:17980025}.
CC -!- DEVELOPMENTAL STAGE: Detected throughout embryogenesis.
CC {ECO:0000269|PubMed:17521969}.
CC -!- INDUCTION: Up-regulated by FGF signaling in embryos at mid-
CC gastrulation. {ECO:0000269|PubMed:17521969}.
CC -!- SIMILARITY: Belongs to the Churchill family. {ECO:0000305}.
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DR EMBL; AL954831; CAI20620.1; -; Genomic_DNA.
DR EMBL; BC085457; AAH85457.1; -; mRNA.
DR RefSeq; NP_001007381.1; NM_001007380.1.
DR AlphaFoldDB; Q5U3N7; -.
DR SMR; Q5U3N7; -.
DR STRING; 7955.ENSDARP00000022916; -.
DR PaxDb; Q5U3N7; -.
DR GeneID; 492508; -.
DR KEGG; dre:492508; -.
DR CTD; 91612; -.
DR ZFIN; ZDB-GENE-041001-150; churc1.
DR eggNOG; ENOG502S4AE; Eukaryota.
DR InParanoid; Q5U3N7; -.
DR OrthoDB; 1549523at2759; -.
DR PhylomeDB; Q5U3N7; -.
DR TreeFam; TF333004; -.
DR PRO; PR:Q5U3N7; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0042074; P:cell migration involved in gastrulation; IMP:ZFIN.
DR GO; GO:0008543; P:fibroblast growth factor receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0090243; P:fibroblast growth factor receptor signaling pathway involved in somitogenesis; IMP:ZFIN.
DR GO; GO:0030336; P:negative regulation of cell migration; IMP:ZFIN.
DR GO; GO:0040037; P:negative regulation of fibroblast growth factor receptor signaling pathway; IMP:ZFIN.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0030334; P:regulation of cell migration; IMP:ZFIN.
DR GO; GO:0042661; P:regulation of mesodermal cell fate specification; IMP:ZFIN.
DR Gene3D; 2.60.40.4240; -; 1.
DR InterPro; IPR038543; Churchill_sf.
DR InterPro; IPR009508; Transcrpt_activator_Churchill.
DR PANTHER; PTHR31931; PTHR31931; 1.
DR Pfam; PF06573; Churchill; 1.
PE 2: Evidence at transcript level;
KW Activator; Developmental protein; Metal-binding; Reference proteome;
KW Transcription; Transcription regulation; Zinc.
FT CHAIN 1..112
FT /note="Protein Churchill"
FT /id="PRO_0000351552"
FT BINDING 2
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 5
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 30
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 30
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 33
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 59
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 61
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 64
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 66
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 71
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 88
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 91
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT CONFLICT 100
FT /note="V -> I (in Ref. 1; CAI20620)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 112 AA; 12904 MW; 5288321CF545BC55 CRC64;
MCTGCVQKQY PDRGNTCLEN GSYLMNFVGC ANCHQRDFVL ISDRTMVNED EEEIVTYLHM
CKNCEHVIAR HEYTFTVVDD YQEYTMLCML CGKAEDSISV LPDDPRQTAP LF
