CHUR_HUMAN
ID CHUR_HUMAN Reviewed; 112 AA.
AC Q8WUH1; A0A0C4DGJ7; B3KQ81; G3V1X3; G3V214; Q9H3K7;
DT 30-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 23-FEB-2022, sequence version 3.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Protein Churchill;
GN Name=CHURC1; Synonyms=C14orf52, CHCH; ORFNames=My015;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Fetal brain;
RA Mao Y.M., Xie Y., Lin Q., Li Y., Dai J.L., Ying K.;
RL Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Trachea;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12508121; DOI=10.1038/nature01348;
RA Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C.,
RA Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A.,
RA Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H.,
RA Du H., Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T.,
RA Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B.,
RA Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D.,
RA Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R.,
RA Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S.,
RA Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C.,
RA Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S.,
RA Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C.,
RA Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P.,
RA Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M.,
RA Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V.,
RA Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J.,
RA Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F.,
RA Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F.,
RA Waterston R., Hood L., Weissenbach J.;
RT "The DNA sequence and analysis of human chromosome 14.";
RL Nature 421:601-607(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP STRUCTURE BY NMR OF 2-109 IN COMPLEX WITH ZINC IONS, AND FUNCTION (ISOFORM
RP 4).
RX PubMed=17610897; DOI=10.1016/j.jmb.2007.06.021;
RA Lee B.M., Buck-Koehntop B.A., Martinez-Yamout M.A., Dyson H.J.,
RA Wright P.E.;
RT "Embryonic neural inducing factor Churchill is not a DNA-binding zinc
RT finger protein: solution structure reveals a solvent-exposed beta-sheet and
RT zinc binuclear cluster.";
RL J. Mol. Biol. 371:1274-1289(2007).
CC -!- FUNCTION: Transcriptional activator that mediates FGF signaling during
CC neural development (By similarity). Plays a role in the regulation of
CC cell movement (By similarity). {ECO:0000250|UniProtKB:Q5U3N7,
CC ECO:0000250|UniProtKB:Q9DFZ3}.
CC -!- FUNCTION: [Isoform 4]: Does not bind DNA by itself.
CC {ECO:0000269|PubMed:17610897}.
CC -!- INTERACTION:
CC Q8WUH1; Q9P0T4: ZNF581; NbExp=3; IntAct=EBI-11156883, EBI-745520;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing, Alternative initiation; Named isoforms=4;
CC Name=4;
CC IsoId=Q8WUH1-4; Sequence=Displayed;
CC Name=1;
CC IsoId=Q8WUH1-1; Sequence=VSP_061409;
CC Name=2;
CC IsoId=Q8WUH1-2; Sequence=VSP_061411;
CC Name=3;
CC IsoId=Q8WUH1-3; Sequence=VSP_061410, VSP_061412;
CC -!- SIMILARITY: Belongs to the Churchill family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG43128.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF060510; AAG43128.1; ALT_FRAME; mRNA.
DR EMBL; AK057626; BAG51943.1; -; mRNA.
DR EMBL; AL135745; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL139022; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; KF455908; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471061; EAW80884.1; -; Genomic_DNA.
DR EMBL; CH471061; EAW80887.1; -; Genomic_DNA.
DR EMBL; BC020550; AAH20550.1; -; mRNA.
DR CCDS; CCDS32101.2; -. [Q8WUH1-2]
DR CCDS; CCDS55921.1; -. [Q8WUH1-4]
DR CCDS; CCDS55922.1; -. [Q8WUH1-3]
DR RefSeq; NP_001190992.1; NM_001204063.1. [Q8WUH1-1]
DR RefSeq; NP_001190993.1; NM_001204064.1. [Q8WUH1-3]
DR RefSeq; NP_660148.3; NM_145165.3. [Q8WUH1-2]
DR PDB; 2JOX; NMR; -; A=2-107.
DR PDBsum; 2JOX; -.
DR AlphaFoldDB; Q8WUH1; -.
DR BMRB; Q8WUH1; -.
DR SMR; Q8WUH1; -.
DR BioGRID; 124853; 19.
DR IntAct; Q8WUH1; 6.
DR MINT; Q8WUH1; -.
DR STRING; 9606.ENSP00000475473; -.
DR iPTMnet; Q8WUH1; -.
DR PhosphoSitePlus; Q8WUH1; -.
DR BioMuta; CHURC1; -.
DR DMDM; 510120833; -.
DR EPD; Q8WUH1; -.
DR jPOST; Q8WUH1; -.
DR MassIVE; Q8WUH1; -.
DR MaxQB; Q8WUH1; -.
DR PaxDb; Q8WUH1; -.
DR PeptideAtlas; Q8WUH1; -.
DR PRIDE; Q8WUH1; -.
DR ProteomicsDB; 32466; -.
DR ProteomicsDB; 32497; -.
DR Antibodypedia; 51632; 60 antibodies from 19 providers.
DR DNASU; 91612; -.
DR Ensembl; ENST00000548752.7; ENSP00000450165.3; ENSG00000258289.10. [Q8WUH1-3]
DR Ensembl; ENST00000549115.7; ENSP00000448050.2; ENSG00000258289.10. [Q8WUH1-4]
DR Ensembl; ENST00000552002.7; ENSP00000450144.2; ENSG00000258289.10. [Q8WUH1-4]
DR Ensembl; ENST00000607599.6; ENSP00000475473.2; ENSG00000258289.10. [Q8WUH1-2]
DR GeneID; 91612; -.
DR KEGG; hsa:91612; -.
DR MANE-Select; ENST00000549115.7; ENSP00000448050.2; NM_001386928.1; NP_001373857.1.
DR UCSC; uc001xhv.4; human. [Q8WUH1-4]
DR CTD; 91612; -.
DR DisGeNET; 91612; -.
DR GeneCards; CHURC1; -.
DR HGNC; HGNC:20099; CHURC1.
DR HPA; ENSG00000258289; Low tissue specificity.
DR MIM; 608577; gene.
DR neXtProt; NX_Q8WUH1; -.
DR OpenTargets; ENSG00000258289; -.
DR PharmGKB; PA134982480; -.
DR VEuPathDB; HostDB:ENSG00000258289; -.
DR eggNOG; ENOG502S4AE; Eukaryota.
DR GeneTree; ENSGT00390000011163; -.
DR HOGENOM; CLU_142022_0_0_1; -.
DR InParanoid; Q8WUH1; -.
DR TreeFam; TF333004; -.
DR PathwayCommons; Q8WUH1; -.
DR SignaLink; Q8WUH1; -.
DR BioGRID-ORCS; 91612; 18 hits in 1055 CRISPR screens.
DR EvolutionaryTrace; Q8WUH1; -.
DR GenomeRNAi; 91612; -.
DR Pharos; Q8WUH1; Tbio.
DR PRO; PR:Q8WUH1; -.
DR Proteomes; UP000005640; Chromosome 14.
DR RNAct; Q8WUH1; protein.
DR Bgee; ENSG00000258289; Expressed in calcaneal tendon and 186 other tissues.
DR ExpressionAtlas; Q8WUH1; baseline and differential.
DR Genevisible; Q8WUH1; HS.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0008543; P:fibroblast growth factor receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 2.60.40.4240; -; 1.
DR InterPro; IPR038543; Churchill_sf.
DR InterPro; IPR009508; Transcrpt_activator_Churchill.
DR PANTHER; PTHR31931; PTHR31931; 1.
DR Pfam; PF06573; Churchill; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Alternative initiation; Alternative splicing;
KW Developmental protein; Metal-binding; Reference proteome; Transcription;
KW Transcription regulation; Zinc.
FT CHAIN 1..112
FT /note="Protein Churchill"
FT /id="PRO_0000089665"
FT BINDING 2
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT BINDING 5
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT BINDING 30
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT BINDING 30
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT BINDING 33
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT BINDING 59
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="3"
FT BINDING 61
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT BINDING 64
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT BINDING 66
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT BINDING 71
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="3"
FT BINDING 88
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="3"
FT BINDING 91
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="3"
FT VAR_SEQ 1
FT /note="M -> MRQPYLSSREVSSSRKRWRTFPVDCVAM (in isoform 1)"
FT /id="VSP_061409"
FT VAR_SEQ 59..78
FT /note="HLCKNCHHVIARHEYTFSIM -> RVYHAVSVMRQSRRYYQYSP (in
FT isoform 3)"
FT /id="VSP_061410"
FT VAR_SEQ 59
FT /note="H -> PD (in isoform 2)"
FT /id="VSP_061411"
FT VAR_SEQ 79..112
FT /note="Missing (in isoform 3)"
FT /id="VSP_061412"
FT STRAND 6..10
FT /evidence="ECO:0007829|PDB:2JOX"
FT TURN 24..26
FT /evidence="ECO:0007829|PDB:2JOX"
FT STRAND 31..33
FT /evidence="ECO:0007829|PDB:2JOX"
FT STRAND 39..49
FT /evidence="ECO:0007829|PDB:2JOX"
FT STRAND 52..61
FT /evidence="ECO:0007829|PDB:2JOX"
FT TURN 62..64
FT /evidence="ECO:0007829|PDB:2JOX"
FT STRAND 67..78
FT /evidence="ECO:0007829|PDB:2JOX"
FT STRAND 81..88
FT /evidence="ECO:0007829|PDB:2JOX"
FT TURN 89..91
FT /evidence="ECO:0007829|PDB:2JOX"
FT STRAND 92..99
FT /evidence="ECO:0007829|PDB:2JOX"
SQ SEQUENCE 112 AA; 12887 MW; C817115321C29EC3 CRC64;
MCGDCVEKEY PNRGNTCLEN GSFLLNFTGC AVCSKRDFML ITNKSLKEED GEEIVTYDHL
CKNCHHVIAR HEYTFSIMDE FQEYTMLCLL CGKAEDTISI LPDDPRQMTL LF
