CHVG_RHIME
ID CHVG_RHIME Reviewed; 577 AA.
AC P72292; O31069;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2001, sequence version 3.
DT 25-MAY-2022, entry version 145.
DE RecName: Full=Sensor protein ChvG;
DE EC=2.7.13.3;
DE AltName: Full=Histidine kinase sensory protein ExoS;
GN Name=chvG; Synonyms=exoS; OrderedLocusNames=R00043; ORFNames=SMc04446;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=9422587; DOI=10.1128/jb.180.1.20-26.1998;
RA Cheng H.-P., Walker G.C.;
RT "Succinoglycan production by Rhizobium meliloti is regulated through the
RT ExoS-ChvI two-component regulatory system.";
RL J. Bacteriol. 180:20-26(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481430; DOI=10.1073/pnas.161294398;
RA Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT meliloti strain 1021.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-112.
RC STRAIN=SU47 / 1021;
RX PubMed=7559334; DOI=10.1128/jb.177.19.5485-5494.1995;
RA Oesteraas M., Stanley J., Finan T.M.;
RT "Identification of Rhizobium-specific intergenic mosaic elements within an
RT essential two-component regulatory system of Rhizobium species.";
RL J. Bacteriol. 177:5485-5494(1995).
CC -!- FUNCTION: Member of a two-component regulatory system ChvG(ExoS)/ChvI
CC involved in regulating the production of succinoglycan. Activates ChvI
CC by phosphorylation.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC histidine.; EC=2.7.13.3;
CC -!- PATHWAY: Glycan metabolism; exopolysaccharide biosynthesis.
CC -!- SUBUNIT: Homodimer.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAC41430.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF027298; AAB96631.1; -; Genomic_DNA.
DR EMBL; AL591688; CAC41430.1; ALT_INIT; Genomic_DNA.
DR EMBL; U32941; AAB07686.2; -; Genomic_DNA.
DR RefSeq; NP_384149.1; NC_003047.1.
DR AlphaFoldDB; P72292; -.
DR SMR; P72292; -.
DR STRING; 266834.SMc04446; -.
DR EnsemblBacteria; CAC41430; CAC41430; SMc04446.
DR KEGG; sme:SMc04446; -.
DR PATRIC; fig|266834.11.peg.1397; -.
DR eggNOG; COG2205; Bacteria.
DR HOGENOM; CLU_000445_89_6_5; -.
DR BRENDA; 2.7.13.3; 5347.
DR UniPathway; UPA00631; -.
DR PRO; PR:P72292; -.
DR Proteomes; UP000001976; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR GO; GO:0000271; P:polysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR CDD; cd00082; HisKA; 1.
DR Gene3D; 3.30.565.10; -; 1.
DR InterPro; IPR003660; HAMP_dom.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR005467; His_kinase_dom.
DR InterPro; IPR003661; HisK_dim/P.
DR InterPro; IPR036097; HisK_dim/P_sf.
DR InterPro; IPR025908; Sensor_TM1.
DR InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR InterPro; IPR025919; Stimulus_sens_dom.
DR Pfam; PF00672; HAMP; 1.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF00512; HisKA; 1.
DR Pfam; PF13755; Sensor_TM1; 1.
DR Pfam; PF13756; Stimulus_sens_1; 1.
DR PRINTS; PR00344; BCTRLSENSOR.
DR SMART; SM00304; HAMP; 1.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00388; HisKA; 1.
DR SUPFAM; SSF47384; SSF47384; 1.
DR SUPFAM; SSF55874; SSF55874; 1.
DR PROSITE; PS50885; HAMP; 1.
DR PROSITE; PS50109; HIS_KIN; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell inner membrane; Cell membrane;
KW Exopolysaccharide synthesis; Kinase; Membrane; Nucleotide-binding;
KW Phosphoprotein; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix; Two-component regulatory system.
FT CHAIN 1..577
FT /note="Sensor protein ChvG"
FT /id="PRO_0000074721"
FT TOPO_DOM 1..29
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 30..50
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 51..260
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 261..281
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 282..577
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 283..339
FT /note="HAMP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00102"
FT DOMAIN 347..575
FT /note="Histidine kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT MOD_RES 350
FT /note="Phosphohistidine"
FT /evidence="ECO:0000250"
FT CONFLICT 136
FT /note="L -> F (in Ref. 1; AAB96631)"
FT /evidence="ECO:0000305"
FT CONFLICT 266
FT /note="V -> A (in Ref. 1; AAB96631)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 577 AA; 63721 MW; BEF2DD4A4F0E2B15 CRC64;
MRGQRRWAHP FTLIRRLFGN AVFSSLTRRI VFFNLVALVV LVGGIMYLNQ FREGLIDARV
ESLLTQGEII AGAISASASV DTNSITIDPE KLLELQAGES ITPLPSDEDL EFPIIQERVA
PVLRRLISPT RTRARLFDAD ADLLLDSRHL YSGGQVLRFD LPPVDPESPS LADEFGTWFN
RLLQPGDLPL YKEPPGGNGS IYPEVMNALT GVRGAVVRVT EKGELIVSVA VPVQRFRAVL
GVLLLSTQAG DIDKIVHAER LAIIRVFGVA ALVNVILSLL LSSTIANPLR RLSAAAIRVR
RGGAKEREEI PDFSSRQDEI GNLSVALREM TTALYDRIAA IENFAADVSH ELKNPLTSLR
SAVETLPLAR NEESKKRLMD VIQHDVRRLD RLISDISDAS RLDAELARAD AKKVDLEKLL
GDLVEISRQI RGSKKPVLLD FVVDRKDNPR ASFIVSGYEL RIGQIITNLI ENARSFVPEQ
NGRIVVRLTR SRLRCIVYVE DNGPGIQAED IDRIFERFYT DRPEGEDFGQ NSGLGLSISR
QIAEAHGGTL RAENIAGKDG RISGARFVLS LPAGPHP
