ID   25KD_SARPE              Reviewed;         258 AA.
AC   P23170;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Development-specific 25 kDa protein;
OS   Sarcophaga peregrina (Flesh fly) (Boettcherisca peregrina).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Oestroidea;
OC   Sarcophagidae; Sarcophaga; Boettcherisca.
OX   NCBI_TaxID=7386;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2993269; DOI=10.1093/oxfordjournals.jbchem.a135205;
RA   Matsumoto N., Sekimizu K., Soma G., Ohmura Y., Andoh T., Nakanishi Y.,
RA   Obinata M., Natori S.;
RT   "Structural analysis of a developmentally regulated 25-kDa protein gene of
RT   Sarcophaga peregrina.";
RL   J. Biochem. 97:1501-1508(1985).
RN   [2]
RP   SEQUENCE REVISION.
RX   PubMed=8647115; DOI=10.1111/j.1432-1033.1996.0698p.x;
RA   Horio T., Kubo T., Natori S.;
RT   "Purification and cDNA cloning of the alcohol dehydrogenase of the flesh
RT   fly Sarcophaga peregrina. A structural relationship between alcohol
RT   dehydrogenase and a 25-kDa protein.";
RL   Eur. J. Biochem. 237:698-703(1996).
CC   -!- DEVELOPMENTAL STAGE: Expressed in the fat body of middle third-instar
CC       larvae.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X02570; CAA26412.1; -; Genomic_DNA.
DR   PIR; A24181; A24181.
DR   PIR; T11741; T11741.
DR   AlphaFoldDB; P23170; -.
DR   SMR; P23170; -.
DR   GO; GO:0004022; F:alcohol dehydrogenase (NAD+) activity; IEA:InterPro.
DR   InterPro; IPR002426; ADH_Ceratitis-type.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR01169; CERATITISADH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   2: Evidence at transcript level;
KW   Oxidoreductase.
FT   CHAIN           1..258
FT                   /note="Development-specific 25 kDa protein"
FT                   /id="PRO_0000054445"
FT   ACT_SITE        151
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         10..34
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         138
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   258 AA;  28633 MW;  651CB188A5D1547A CRC64;
     MMDWNNKNVV YVGGFSGFGY QVCQMMMKKP MKHLIVCSRM ENVEMLKKLQ AINTSVKVMF
     VQMNIADYAS IVKGVKQVIG HVGHVDVLIN GVGGLADKDV ETTVAVNLTG LINTTLMFMP
     YMDKTQSGHG GMVVSISSVY GLEPGPAFSV YSAAKHGGIG FTRSMADEHL YHKTGVAFMC
     ICPAMTSTEL MMNKRDMNWM KWVPHSEEMW KMVMDAKMQT PEECAVNMMT AMEQAKNGAI
     YICSTSGMKE ITPTVYMH