CHX13_ARATH
ID CHX13_ARATH Reviewed; 831 AA.
AC O22920; Q58P68;
DT 16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 122.
DE RecName: Full=Cation/H(+) symporter 13;
DE AltName: Full=Protein CATION/H+ EXCHANGER 13;
DE Short=AtCHX13;
GN Name=CHX13; OrderedLocusNames=At2g30240; ORFNames=T27E13, T9D9.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, GENE FAMILY, AND
RP NOMENCLATURE.
RC TISSUE=Pollen;
RX PubMed=15347787; DOI=10.1104/pp.104.046003;
RA Sze H., Padmanaban S., Cellier F., Honys D., Cheng N.-H., Bock K.W.,
RA Conejero G., Li X., Twell D., Ward J.M., Hirschi K.D.;
RT "Expression patterns of a novel AtCHX gene family highlight potential roles
RT in osmotic adjustment and K+ homeostasis in pollen development.";
RL Plant Physiol. 136:2532-2547(2004).
RN [2]
RP SEQUENCE REVISION, SUBCELLULAR LOCATION, INDUCTION, BIOPHYSICOCHEMICAL
RP PROPERTIES, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=18676662; DOI=10.1104/pp.108.124248;
RA Zhao J., Cheng N.-H., Motes C.M., Blancaflor E.B., Moore M., Gonzales N.,
RA Padmanaban S., Sze H., Ward J.M., Hirschi K.D.;
RT "AtCHX13 is a plasma membrane K+ transporter.";
RL Plant Physiol. 148:796-807(2008).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
CC -!- FUNCTION: High-affinity potassium transporter that plays a role in K(+)
CC acquisition. May operate as a K(+)/H(+) symporter.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=196 uM for K(+) {ECO:0000269|PubMed:18676662};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:18676662};
CC Multi-pass membrane protein {ECO:0000269|PubMed:18676662}.
CC -!- TISSUE SPECIFICITY: Preferentially expressed in pollen before and after
CC germination. Detected in pollen grains within anthers of the flower
CC buds or in pollen on fully open flowers and on the stigma, and in
CC pollen tubes growing in the style. Weakly expressed in roots.
CC {ECO:0000269|PubMed:15347787}.
CC -!- INDUCTION: Transiently induced by K(+) depletion.
CC {ECO:0000269|PubMed:18676662}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under standard growth
CC conditions, but growth inhibition and leaf chlorosis and bleaching
CC under low K(+) conditions. {ECO:0000269|PubMed:18676662}.
CC -!- MISCELLANEOUS: K(+) uptake enhanced by a proton electrochemical
CC gradient (acidic outside). Not affected by specific inhibitors of
CC voltage-gated K(+) channels.
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 2 (CPA2)
CC transporter (TC 2.A.37) family. CHX (TC 2.A.37.4) subfamily.
CC {ECO:0000305}.
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DR EMBL; EF571901; AAX49543.2; -; mRNA.
DR EMBL; AC002338; AAC16929.1; -; Genomic_DNA.
DR EMBL; AC004165; AAM14917.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08361.1; -; Genomic_DNA.
DR PIR; T00576; T00576.
DR RefSeq; NP_180583.1; NM_128577.2.
DR AlphaFoldDB; O22920; -.
DR SMR; O22920; -.
DR STRING; 3702.AT2G30240.1; -.
DR TCDB; 2.A.37.4.3; the monovalent cation:proton antiporter-2 (cpa2) family.
DR iPTMnet; O22920; -.
DR MetOSite; O22920; -.
DR PaxDb; O22920; -.
DR PRIDE; O22920; -.
DR ProteomicsDB; 246969; -.
DR EnsemblPlants; AT2G30240.1; AT2G30240.1; AT2G30240.
DR GeneID; 817574; -.
DR Gramene; AT2G30240.1; AT2G30240.1; AT2G30240.
DR KEGG; ath:AT2G30240; -.
DR Araport; AT2G30240; -.
DR TAIR; locus:2060827; AT2G30240.
DR eggNOG; KOG1650; Eukaryota.
DR HOGENOM; CLU_005126_6_2_1; -.
DR InParanoid; O22920; -.
DR OMA; LMTNTWQ; -.
DR OrthoDB; 336706at2759; -.
DR PhylomeDB; O22920; -.
DR SABIO-RK; O22920; -.
DR PRO; PR:O22920; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O22920; baseline and differential.
DR Genevisible; O22920; AT.
DR GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015079; F:potassium ion transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015299; F:solute:proton antiporter activity; IEA:InterPro.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006885; P:regulation of pH; IBA:GO_Central.
DR Gene3D; 1.20.1530.20; -; 1.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR Pfam; PF00999; Na_H_Exchanger; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Ion transport; Membrane; Potassium; Potassium transport;
KW Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..831
FT /note="Cation/H(+) symporter 13"
FT /id="PRO_0000378329"
FT TRANSMEM 50..70
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..234
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 250..270
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 303..323
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 334..354
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 364..384
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 397..417
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 430..450
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 831 AA; 92189 MW; 55F767EF718FF470 CRC64;
MELSMFGRED TNSSQWGEVH GPFMLEEIVC QAQNMLTSRG IFMKSNPLKY ALPLLLLQMS
VIIVTSRLIF RVLQPLKQGM ISAQVLTGVV LGPSFLGHNV IYMNMFLPAG GKIIIQTLSN
VGFVIHLFLL GLKIDGSIIR KAGSKAILIG TASYAFPFSL GNLTIMFISK TMGLPSDVIS
CTSSAISLSS MTSFPVTTTV LAELNILNSE LGRLATHCSM VCEVCSWFVA LAFNLYTRDR
TMTSLYALSM IIGLLLVIYF VFRPIIVWLT QRKTKSMDKK DVVPFFPVLL LLSIASLSGE
AMGVHAAFGA FWLGVSLPDG PPLGTELAAK LEMFASNLFL PCFIAISGLQ TNFFEITESH
EHHVVMIEII LLITYGCKFL GTAAASAYCQ TQIGDALCLA FLMCCQGIIE VYTTIVWKDA
QVVDTECFNL VIITILFVTG ISRFLVVYLY DPSKRYKSKS KRTILNTRQH NLQLRLLLGL
YNVENVPSMV NLLEATYPTR FNPISFFTLH LVELKGRAHA LLTPHHQMNK LDPNTAQSTH
IVNAFQRFEQ KYQGALMAQH FTAAAPYSSI NNDICTLALD KKATLIVIPF HKQYAIDGTV
GQVNGPIRTI NLNVLDAAPC SVAIFIDRGE TEGRRSVLMT NTWQNVAMLF IGGKDDAEAL
ALCMRMAEKP DLNVTMIHFR HKSALQDEDY SDMSEYNLIS DFKSYAANKG KIHYVEEIVR
DGVETTQVIS SLGDAYDMVL VGRDHDLESS VLYGLTDWSE CPELGVIGDM LTSPDFHFSV
LVVHQQQGDD LLAMDDSYKL PNVEHQKIGD TGIQQRFSAE EGFTTIDLGK R
