CHX27_ARATH
ID CHX27_ARATH Reviewed; 767 AA.
AC Q9M007; F4K9H0;
DT 15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT 15-JUN-2010, sequence version 2.
DT 25-MAY-2022, entry version 94.
DE RecName: Full=Cation/H(+) antiporter 27;
DE AltName: Full=Protein CATION/H+ EXCHANGER 27;
DE Short=AtCHX27;
GN Name=CHX27; OrderedLocusNames=At5g01690; ORFNames=F7A7_210;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
RN [4]
RP TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15347787; DOI=10.1104/pp.104.046003;
RA Sze H., Padmanaban S., Cellier F., Honys D., Cheng N.-H., Bock K.W.,
RA Conejero G., Li X., Twell D., Ward J.M., Hirschi K.D.;
RT "Expression patterns of a novel AtCHX gene family highlight potential roles
RT in osmotic adjustment and K+ homeostasis in pollen development.";
RL Plant Physiol. 136:2532-2547(2004).
CC -!- FUNCTION: May operate as a cation/H(+) antiporter. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in pollen.
CC {ECO:0000269|PubMed:15347787}.
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 2 (CPA2)
CC transporter (TC 2.A.37) family. CHX (TC 2.A.37.4) subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB82285.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL161946; CAB82285.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED90377.2; -; Genomic_DNA.
DR PIR; T48190; T48190.
DR RefSeq; NP_001318455.1; NM_001342606.1.
DR AlphaFoldDB; Q9M007; -.
DR SMR; Q9M007; -.
DR BioGRID; 15739; 12.
DR IntAct; Q9M007; 12.
DR PaxDb; Q9M007; -.
DR PRIDE; Q9M007; -.
DR ProteomicsDB; 246884; -.
DR EnsemblPlants; AT5G01690.1; AT5G01690.1; AT5G01690.
DR GeneID; 830460; -.
DR Gramene; AT5G01690.1; AT5G01690.1; AT5G01690.
DR KEGG; ath:AT5G01690; -.
DR Araport; AT5G01690; -.
DR TAIR; locus:2149760; AT5G01690.
DR eggNOG; KOG1650; Eukaryota.
DR InParanoid; Q9M007; -.
DR OMA; FICYITT; -.
DR OrthoDB; 336706at2759; -.
DR PhylomeDB; Q9M007; -.
DR PRO; PR:Q9M007; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9M007; baseline and differential.
DR GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW.
DR GO; GO:0006885; P:regulation of pH; IBA:GO_Central.
DR Gene3D; 1.20.1530.20; -; 1.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
PE 2: Evidence at transcript level;
KW Antiport; Ion transport; Membrane; Potassium; Potassium transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..767
FT /note="Cation/H(+) antiporter 27"
FT /id="PRO_0000394996"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 63..83
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..119
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 173..193
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 205..225
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 280..300
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 323..343
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 371..391
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 415..435
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 767 AA; 86991 MW; 5306D4E28E628F0D CRC64;
MENITKTFQY GGVDWLCEPW VGAGSLGIGR GENPLKFALP LLLLQISVFS IFSVSFQFLL
RPFGKFAFLT QMLAGICLGP SVIGRNKQYM ATFFYARSVY IIESFEAICF LFICYITTCQ
VDTRMIKRVG KLAFINGILL FLIPFVWGQF AAILISKRLK SGPAGIPPVE FHHVAIVQST
MFFQVVYGVL SSLKMLNTEP GRLALASMMV HDCLSWCFFM LNIAIKLNVD LPNKNRAAFL
SVLQMIMILV IAYVFRPLML WMKNRTPEGH SLKASYLSVI CVLLFISCLW AEFVGLPYFF
GAVVLGLATP KRPPLGTGLS DKIGCFVWSV LMPCYVIGIG LNIDLSLFSW RDVIRFELLF
GVVRFAKMIA IALPSLYYKV PLWHAILVGF IVNIQGLYDV QIYKQNFNYT KISSKSFGAM
VMSATVNSTI FIVIVKKLYQ TMSKRNPYKR RTVQHCRVEA PLRILTCFRN REAVRPVLDL
VELSRPAIGS PLSVFAVNLE ELNNHSLPLL IHHTQEISPF LVPSRRDQIV KAFHNFEKTN
QETVLIECFT AVAPRKTMHE DVCAIAFDQE TDIVILTLDA GIELWERLLC RNLLHNCPCS
VALFIDRGRL PDFRFVPLKK LTINIGAIFL GGPDDREMLA YATRLASHPS VELQVFRLVD
QNGVSPLRDM VERNHDMRVI NVFRKENSEK NIIFREVRIE EAVNLLDLLR KEGDDFDLMM
VGIRHEENLL MLEGLSEWSD MKELGEVGDV LISKDLELSV SVLAVQQ
