CHX28_ARATH
ID CHX28_ARATH Reviewed; 801 AA.
AC Q8L709; Q9SUZ6;
DT 15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT 15-JUN-2010, sequence version 2.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Cation/H(+) antiporter 28;
DE AltName: Full=Protein CATION/H+ EXCHANGER 28;
DE Short=AtCHX28;
GN Name=CHX28; OrderedLocusNames=At3g52080; ORFNames=F4F15.190;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
RN [5]
RP TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15347787; DOI=10.1104/pp.104.046003;
RA Sze H., Padmanaban S., Cellier F., Honys D., Cheng N.-H., Bock K.W.,
RA Conejero G., Li X., Twell D., Ward J.M., Hirschi K.D.;
RT "Expression patterns of a novel AtCHX gene family highlight potential roles
RT in osmotic adjustment and K+ homeostasis in pollen development.";
RL Plant Physiol. 136:2532-2547(2004).
CC -!- FUNCTION: May operate as a cation/H(+) antiporter. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in pollen.
CC {ECO:0000269|PubMed:15347787}.
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 2 (CPA2)
CC transporter (TC 2.A.37) family. CHX (TC 2.A.37.4) subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB41328.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL049711; CAB41328.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE78888.1; -; Genomic_DNA.
DR EMBL; AY140034; AAM98175.1; -; mRNA.
DR PIR; T49087; T49087.
DR RefSeq; NP_190776.2; NM_115067.3.
DR AlphaFoldDB; Q8L709; -.
DR SMR; Q8L709; -.
DR BioGRID; 9689; 8.
DR IntAct; Q8L709; 8.
DR PaxDb; Q8L709; -.
DR PRIDE; Q8L709; -.
DR ProteomicsDB; 246842; -.
DR EnsemblPlants; AT3G52080.1; AT3G52080.1; AT3G52080.
DR GeneID; 824371; -.
DR Gramene; AT3G52080.1; AT3G52080.1; AT3G52080.
DR KEGG; ath:AT3G52080; -.
DR Araport; AT3G52080; -.
DR TAIR; locus:2083830; AT3G52080.
DR eggNOG; KOG1650; Eukaryota.
DR HOGENOM; CLU_005126_6_2_1; -.
DR InParanoid; Q8L709; -.
DR OMA; FGMICYM; -.
DR OrthoDB; 336706at2759; -.
DR PhylomeDB; Q8L709; -.
DR PRO; PR:Q8L709; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8L709; baseline and differential.
DR Genevisible; Q8L709; AT.
DR GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015299; F:solute:proton antiporter activity; IEA:InterPro.
DR GO; GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW.
DR GO; GO:0006885; P:regulation of pH; IBA:GO_Central.
DR Gene3D; 1.20.1530.20; -; 1.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR Pfam; PF00999; Na_H_Exchanger; 1.
PE 2: Evidence at transcript level;
KW Antiport; Ion transport; Membrane; Potassium; Potassium transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..801
FT /note="Cation/H(+) antiporter 28"
FT /id="PRO_0000394997"
FT TRANSMEM 24..44
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 275..292
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..324
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..363
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 371..391
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 403..423
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 275
FT /note="S -> L (in Ref. 3; AAM98175)"
FT /evidence="ECO:0000305"
FT CONFLICT 597
FT /note="K -> E (in Ref. 3; AAM98175)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 801 AA; 88710 MW; 93F1A3406C6D5289 CRC64;
MNSTTTKNVC GDKWYLNLDR PEEALKILVF IAIFVVRTLL HYLMKPLGQP YLTTDFAIGL
ILGNIPRFRG AFSGPYSITL NNIIEFGMIC HMFVMGLEMN PSVLLRPPTK DAFIAYTSMI
TTFVLAFVTT PFLHYTKTSP YIFSLALSLM ASSTGSPILT RVIANLKIRK SDLGKLASAA
GVHTDMISTL LYCFGFIFFP TEKPLARPLH RFFRALLMFC LFLAQVTFTS IVSPIFLNWV
NNENPEGKPL KGSHLVMSLA FVVLICSFPT WPPESMYNPI LSAFTAGLFL PNKGRMSKWI
INKINYLLST VFYPIFFFWV GFIIHMRNFD ITDKMAWVRF FSLLGTVIAG KVTGTVLCGL
LLGYHVPETA SLGLLLTTKG HFHVYLAALA IRTNRVKSTT GALIIFIIVF TVVYSPFVVM
DIIKRARKRV PVHIMALQWL DPTTELRILI GLHGPHNIGS TLNVMEICHG GREPGSIFYA
TDMVELTDEI AATLKKGGGA GQSNDSVTVT DRSVTEMRES ITAAVNGYGE LRNGQGVTVR
RMLALSTFVT MAHDVCGLAD ELMVSIIILP FHKRLNPDGT LDAGHAGFRH VNRKILKNAP
CSVGILVDRS FGQTEEAWRP GASMGIAIIF IGGRDDREAL AFAAQVARHP AVKLKVIRFL
EDKSSQNAQK RSSILNRASV VDQEEEMKLD DECFAEFYER YIAGGGRVSY MEKHLTNSSE
TFTALKSLDG EYGLVIVGRG GGRASSGLTT GLNDWQQCPE LGPIGDVLSG SDFSHNTSML
IIQQQRTRGQ LEGLHDDFTI L
