ID   CHX2_ARATH              Reviewed;         783 AA.
AC   Q9SAK8;
DT   15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Cation/H(+) antiporter 2;
DE   AltName: Full=Protein CATION/H+ EXCHANGER 2;
DE            Short=AtCHX2;
GN   Name=CHX2; Synonyms=CHX02; OrderedLocusNames=At1g79400; ORFNames=T8K14.18;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA   Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA   Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA   Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT   "Phylogenetic relationships within cation transporter families of
RT   Arabidopsis.";
RL   Plant Physiol. 126:1646-1667(2001).
RN   [4]
RP   TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=15347787; DOI=10.1104/pp.104.046003;
RA   Sze H., Padmanaban S., Cellier F., Honys D., Cheng N.-H., Bock K.W.,
RA   Conejero G., Li X., Twell D., Ward J.M., Hirschi K.D.;
RT   "Expression patterns of a novel AtCHX gene family highlight potential roles
RT   in osmotic adjustment and K+ homeostasis in pollen development.";
RL   Plant Physiol. 136:2532-2547(2004).
CC   -!- FUNCTION: May operate as a cation/H(+) antiporter. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Specifically expressed in pollen.
CC       {ECO:0000269|PubMed:15347787}.
CC   -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 2 (CPA2)
CC       transporter (TC 2.A.37) family. CHX (TC 2.A.37.4) subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AC007202; AAD30236.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE36238.1; -; Genomic_DNA.
DR   PIR; A96825; A96825.
DR   RefSeq; NP_178058.1; NM_106588.1.
DR   AlphaFoldDB; Q9SAK8; -.
DR   SMR; Q9SAK8; -.
DR   STRING; 3702.AT1G79400.1; -.
DR   PaxDb; Q9SAK8; -.
DR   PRIDE; Q9SAK8; -.
DR   ProteomicsDB; 246843; -.
DR   EnsemblPlants; AT1G79400.1; AT1G79400.1; AT1G79400.
DR   GeneID; 844278; -.
DR   Gramene; AT1G79400.1; AT1G79400.1; AT1G79400.
DR   KEGG; ath:AT1G79400; -.
DR   Araport; AT1G79400; -.
DR   TAIR; locus:2206360; AT1G79400.
DR   eggNOG; KOG1650; Eukaryota.
DR   HOGENOM; CLU_005126_6_2_1; -.
DR   InParanoid; Q9SAK8; -.
DR   OMA; VISHFFH; -.
DR   OrthoDB; 336706at2759; -.
DR   PhylomeDB; Q9SAK8; -.
DR   PRO; PR:Q9SAK8; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9SAK8; baseline and differential.
DR   Genevisible; Q9SAK8; AT.
DR   GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015299; F:solute:proton antiporter activity; IEA:InterPro.
DR   GO; GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0006885; P:regulation of pH; IBA:GO_Central.
DR   Gene3D; 1.20.1530.20; -; 1.
DR   InterPro; IPR006153; Cation/H_exchanger.
DR   InterPro; IPR038770; Na+/solute_symporter_sf.
DR   Pfam; PF00999; Na_H_Exchanger; 1.
PE   2: Evidence at transcript level;
KW   Antiport; Ion transport; Membrane; Potassium; Potassium transport;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..783
FT                   /note="Cation/H(+) antiporter 2"
FT                   /id="PRO_0000394972"
FT   TRANSMEM        19..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        43..63
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        81..101
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        121..141
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        145..165
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        186..206
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        208..228
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        242..262
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        300..320
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        323..343
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        355..375
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        391..411
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   783 AA;  88204 MW;  0A6846910C381648 CRC64;
     MDPKLLLCLP QGDELFNPLN TMFIQMACIL VFSQLFYLLL KPCGQAGPVA QILAGIVLSP
     VLLSRIPKVK EFFLQKNAAD YYSFFSFALR TSFMFLIGLE VDLHFMRRNF KKAAVITLSS
     FVVSGLLSFA SLMLFIPLFG IKEDYFTFFL VLLVTLSNTA SPVVVRSIAD WKLNTCEIGR
     LTISCALFIE LTNVVLYTII MAFISGTIIL ELFLFLLATV ALILINMVLA PWLPKRNPKE
     KYLSKAETLV FFIFLLIIGI TIESYDVNSS VSVFAIGIMF PRQGKTHRTL IQRLSYPIHE
     FVLPVYFGYI GFRFSIIALT KRFYLGIVII VIVTIAGKFI GVISACMYLK IPKKYWLFLP
     TILSVKGHVG LLLLDSNYSE KKWWTTTIHD MMVAALVITT LVSGVLASFL LKTREKDFAY
     EKTSLESHNT NEELRILSCA YGVRHARGAI SLVSALSGSR GASDPFTPLL MHLVPLPKKR
     KSELMYHEHD EDGGNANGDD EFGTNEGLEI NDSIDSFAKD SKILIQQVKL VTQMLNMHEE
     ICNATEDLRV SIVFLPFHKH QRIDGKTTND GELFRQMNRN VLRHGPCSIG IFVDRNITGF
     QQPHGFDSVQ HVATLFFGGP DDREALALCR WLANNTLIHL TVIQFVSEES KAETPVGNAM
     TRDNNEVFME VLGRNQTEQE TDRSFLEEFY NRFVTTGQVG FIEKLVSNGP HTLTILREIG
     EMYSLFVVGK STGDCPMTVR MKDWEECPEL GTVGDFLASS LDVNASVLVV QRQRHSHDSF
     IDD