ID   CHX3_ARATH              Reviewed;         822 AA.
AC   Q9FFB8; Q58P73;
DT   15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Cation/H(+) antiporter 3;
DE   AltName: Full=Protein CATION/H+ EXCHANGER 3;
DE            Short=AtCHX3;
GN   Name=CHX3; Synonyms=CHX03; OrderedLocusNames=At5g22900; ORFNames=MRN17.13;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA   Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA   Miyajima N., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT   features of the 1.6 Mb regions covered by twenty physically assigned P1
RT   clones.";
RL   DNA Res. 4:215-230(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1-811, GENE FAMILY, AND NOMENCLATURE.
RC   TISSUE=Pollen;
RX   PubMed=15347787; DOI=10.1104/pp.104.046003;
RA   Sze H., Padmanaban S., Cellier F., Honys D., Cheng N.-H., Bock K.W.,
RA   Conejero G., Li X., Twell D., Ward J.M., Hirschi K.D.;
RT   "Expression patterns of a novel AtCHX gene family highlight potential roles
RT   in osmotic adjustment and K+ homeostasis in pollen development.";
RL   Plant Physiol. 136:2532-2547(2004).
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA   Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA   Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA   Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT   "Phylogenetic relationships within cation transporter families of
RT   Arabidopsis.";
RL   Plant Physiol. 126:1646-1667(2001).
CC   -!- FUNCTION: May operate as a cation/H(+) antiporter. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 2 (CPA2)
CC       transporter (TC 2.A.37) family. CHX (TC 2.A.37.4) subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AB005243; BAB10611.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED93095.1; -; Genomic_DNA.
DR   EMBL; AY926466; AAX49538.1; -; mRNA.
DR   RefSeq; NP_197681.1; NM_122196.1.
DR   AlphaFoldDB; Q9FFB8; -.
DR   SMR; Q9FFB8; -.
DR   STRING; 3702.AT5G22900.1; -.
DR   PaxDb; Q9FFB8; -.
DR   PRIDE; Q9FFB8; -.
DR   EnsemblPlants; AT5G22900.1; AT5G22900.1; AT5G22900.
DR   GeneID; 832354; -.
DR   Gramene; AT5G22900.1; AT5G22900.1; AT5G22900.
DR   KEGG; ath:AT5G22900; -.
DR   Araport; AT5G22900; -.
DR   TAIR; locus:2172631; AT5G22900.
DR   eggNOG; KOG1650; Eukaryota.
DR   HOGENOM; CLU_005126_6_1_1; -.
DR   InParanoid; Q9FFB8; -.
DR   OMA; NINVHFW; -.
DR   OrthoDB; 336706at2759; -.
DR   PhylomeDB; Q9FFB8; -.
DR   PRO; PR:Q9FFB8; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FFB8; differential.
DR   Genevisible; Q9FFB8; AT.
DR   GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015299; F:solute:proton antiporter activity; IEA:InterPro.
DR   GO; GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0006885; P:regulation of pH; IBA:GO_Central.
DR   Gene3D; 1.20.1530.20; -; 1.
DR   InterPro; IPR006153; Cation/H_exchanger.
DR   InterPro; IPR038770; Na+/solute_symporter_sf.
DR   Pfam; PF00999; Na_H_Exchanger; 1.
PE   2: Evidence at transcript level;
KW   Antiport; Ion transport; Membrane; Potassium; Potassium transport;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..822
FT                   /note="Cation/H(+) antiporter 3"
FT                   /id="PRO_0000394973"
FT   TRANSMEM        55..75
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        116..136
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        150..170
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        190..210
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        224..244
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        274..294
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        305..325
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        331..351
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        362..382
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        388..408
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        418..438
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        447..467
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   822 AA;  92453 MW;  674E2D96BBEF1F29 CRC64;
     MEVFGDNKMF YMRDTWREGT MICDVLPINP SSNGVWPQQK FSDPNINVHF WNYAFPHLQM
     IFLIISFLWQ FLHFFLRRLG MIRFTSHMLT GVLLSKSFLK ENSAARRFFS TEDYKEIVFS
     LTAACSYMMF WFLMGVKMDT GLIRTTGRKA ITIGLSSVLL STLVCSVIFF GNLRDVGTKN
     SDHTLNSLEY VVIYSIQCLS SFPVVGNLLF ELRLQNSELG RLAISSAVIS DFSTSILASV
     LIFMKELKDE QTRLGSVFIG DVIAGNRPLM RAGIVVLFVC IAIYVFRPLM FYIIKQTPSG
     RPVKAIYLST IIVMVSGSAI LANWCKQSIF MGPFILGLAV PHGPPLGSAI IQKYESAIFG
     TFLPFFIASS STEIDISALF GWEGLNGIIL IMVTSFVVKF IFTTVPALFY GMPMEDCFAL
     SLIMSFKGIF ELGAYALAYQ RGSVRPETFT VACLYITLNS AIIPPILRYL YDPSRMYAGY
     EKRNMQHLKP NSELRILSCI YRTDDISPMI NLLEAICPSR ESPVATYVLH LMELVGQANP
     IFISHKLQTR RTEETSYSNN VLVSFEKFRK DFYGSVFVST YTALSMPDTM HGDICMLALN
     NTTSLILLPF HQTWSADGSA LISNNNMIRN LNKSVLDVAP CSVGVFVYRS SSGRKNISSG
     RKTINGTVPN LSSYNICMIF LGGKDDREAV TLATRMARDP RINITIVRLI TTDEKARENT
     VWDKMLDDEL LRDVKSNTLV DIFYSEKAIE DAAETSSLLR SMVSDFDMFI VGRGNGRTSV
     FTEGLEEWSE FKELGIIGDL LTSQDFNCQA SVLVIQQQQL MI