CHX8_ARATH
ID CHX8_ARATH Reviewed; 816 AA.
AC Q58P71; F4IHR1; Q9ZUW0;
DT 15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Cation/H(+) antiporter 8;
DE AltName: Full=Protein CATION/H+ EXCHANGER 8;
DE Short=AtCHX8;
GN Name=CHX8; Synonyms=CHX08; OrderedLocusNames=At2g28180; ORFNames=F24D13.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, GENE FAMILY, AND
RP NOMENCLATURE.
RC TISSUE=Pollen;
RX PubMed=15347787; DOI=10.1104/pp.104.046003;
RA Sze H., Padmanaban S., Cellier F., Honys D., Cheng N.-H., Bock K.W.,
RA Conejero G., Li X., Twell D., Ward J.M., Hirschi K.D.;
RT "Expression patterns of a novel AtCHX gene family highlight potential roles
RT in osmotic adjustment and K+ homeostasis in pollen development.";
RL Plant Physiol. 136:2532-2547(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
CC -!- FUNCTION: May operate as a cation/H(+) antiporter. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in pollen.
CC {ECO:0000269|PubMed:15347787}.
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 2 (CPA2)
CC transporter (TC 2.A.37) family. CHX (TC 2.A.37.4) subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC98448.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY926468; AAX49540.1; -; mRNA.
DR EMBL; AC005851; AAC98448.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC08088.2; -; Genomic_DNA.
DR PIR; G84681; G84681.
DR RefSeq; NP_180385.3; NM_128378.4.
DR AlphaFoldDB; Q58P71; -.
DR SMR; Q58P71; -.
DR BioGRID; 2714; 1.
DR STRING; 3702.AT2G28180.1; -.
DR TCDB; 2.A.37.4.4; the monovalent cation:proton antiporter-2 (cpa2) family.
DR PaxDb; Q58P71; -.
DR PRIDE; Q58P71; -.
DR ProteomicsDB; 246887; -.
DR EnsemblPlants; AT2G28180.1; AT2G28180.1; AT2G28180.
DR GeneID; 817364; -.
DR Gramene; AT2G28180.1; AT2G28180.1; AT2G28180.
DR KEGG; ath:AT2G28180; -.
DR Araport; AT2G28180; -.
DR eggNOG; KOG1650; Eukaryota.
DR HOGENOM; CLU_005126_6_1_1; -.
DR InParanoid; Q58P71; -.
DR OMA; FAYHQAI; -.
DR OrthoDB; 336706at2759; -.
DR PhylomeDB; Q58P71; -.
DR PRO; PR:Q58P71; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q58P71; baseline and differential.
DR Genevisible; Q58P71; AT.
DR GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015299; F:solute:proton antiporter activity; IEA:InterPro.
DR GO; GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW.
DR GO; GO:0006885; P:regulation of pH; IBA:GO_Central.
DR Gene3D; 1.20.1530.20; -; 1.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR Pfam; PF00999; Na_H_Exchanger; 1.
PE 2: Evidence at transcript level;
KW Antiport; Ion transport; Membrane; Potassium; Potassium transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..816
FT /note="Cation/H(+) antiporter 8"
FT /id="PRO_0000394979"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 97..117
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..183
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 227..247
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..317
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..363
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 382..402
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 413..433
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 447..467
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 816 AA; 90956 MW; 7AAC56BE07C65B3A CRC64;
MGGGDISHMS PEVKWIFEMA WYGETVRYDG LICEEHPPKL SSDGIWEKLI IKSAGLYFWQ
YRLPKLEIVI LLVFFLWQGF NILFKKLGLS IPKLSSMMLA GLLLNVLVTL SGENSIIADI
LVTKNRIDVA GCLGSFGFLI FWFLKGVRMD VKRIFKAEAK ARVTGVAAVT FPIVVGFLLF
NLKSAKNRPL TFQEYDVMLL MESITSFSGI ARLLRDLGMN HSSIGRVALS SALVSDIVGL
LLLIANVSRS SATLADGLAI LTEITLFLVI AFAVVRPIMF KIIKRKGEGR PIEDKYIHGV
LVLVCLSCMY WEDLSQFPPL GAFFLGLAIP NGPPIGSALV ERLESFNFGI ILPLFLTAVM
LRTDTTAWKG ALTFFSGDDK KFAVASLVLL IFLLKLSVSV IVPYLYKMPL RDSIILALIM
SHKGIIELSF YLFSLSLKLV TKDTFSILVL SIVLNSLLIP MAIGFLYDPS KQFICYQKRN
LASMKNMGEL KTLVCIHRPD HISSMINLLE ASYQSEDSPL TCYVLHLVEL RGQDVPTLIS
HKVQKLGVGA GNKYSENVIL SFEHFHRSVC SSISIDTFTC IANANHMQDD ICWLALDKAV
TLIILPFHRT WSLDRTSIVS DVEAIRFLNV NVLKQAPCSV GILIERHLVN KKQEPHESLK
VCVIFVGGKD DREALAFAKR MARQENVTLT VLRLLASGKS KDATGWDQML DTVELRELIK
SNNAGMVKEE TSTIYLEQEI LDGADTSMLL RSMAFDYDLF VVGRTCGENH EATKGIENWC
EFEELGVIGD FLASPDFPSK TSVLVVQQQR TVANNN
