CI085_HUMAN
ID CI085_HUMAN Reviewed; 179 AA.
AC Q96MD7; Q5W0N1; Q5W0N3; Q6PJW9; Q86U95;
DT 05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Uncharacterized protein C9orf85;
GN Name=C9orf85;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Small intestine;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC TISSUE=Kidney, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-137 (ISOFORM 2), AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-137 (ISOFORM 2), AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q96MD7-3; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96MD7-1; Sequence=VSP_014430;
CC Name=3;
CC IsoId=Q96MD7-2; Sequence=VSP_014428, VSP_014429;
CC -!- MISCELLANEOUS: [Isoform 3]: May be produced at very low levels due to a
CC premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC decay. {ECO:0000305}.
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DR EMBL; AK057071; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AL138751; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC010179; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BC052375; AAH52375.1; -; mRNA.
DR CCDS; CCDS6639.1; -. [Q96MD7-1]
DR RefSeq; NP_872311.2; NM_182505.3. [Q96MD7-1]
DR AlphaFoldDB; Q96MD7; -.
DR SMR; Q96MD7; -.
DR BioGRID; 126507; 9.
DR STRING; 9606.ENSP00000334289; -.
DR iPTMnet; Q96MD7; -.
DR PhosphoSitePlus; Q96MD7; -.
DR BioMuta; C9orf85; -.
DR DMDM; 68565262; -.
DR jPOST; Q96MD7; -.
DR MassIVE; Q96MD7; -.
DR MaxQB; Q96MD7; -.
DR PaxDb; Q96MD7; -.
DR PeptideAtlas; Q96MD7; -.
DR PRIDE; Q96MD7; -.
DR ProteomicsDB; 77336; -. [Q96MD7-3]
DR ProteomicsDB; 77337; -. [Q96MD7-1]
DR ProteomicsDB; 77338; -. [Q96MD7-2]
DR Antibodypedia; 60695; 60 antibodies from 10 providers.
DR DNASU; 138241; -.
DR Ensembl; ENST00000334731.7; ENSP00000334289.2; ENSG00000155621.15. [Q96MD7-1]
DR Ensembl; ENST00000377031.7; ENSP00000366230.3; ENSG00000155621.15. [Q96MD7-3]
DR Ensembl; ENST00000479413.1; ENSP00000433086.1; ENSG00000155621.15. [Q96MD7-2]
DR GeneID; 138241; -.
DR KEGG; hsa:138241; -.
DR MANE-Select; ENST00000334731.7; ENSP00000334289.2; NM_182505.5; NP_872311.2. [Q96MD7-1]
DR UCSC; uc004ain.4; human. [Q96MD7-3]
DR CTD; 138241; -.
DR GeneCards; C9orf85; -.
DR HGNC; HGNC:28784; C9orf85.
DR HPA; ENSG00000155621; Low tissue specificity.
DR neXtProt; NX_Q96MD7; -.
DR OpenTargets; ENSG00000155621; -.
DR PharmGKB; PA134985322; -.
DR VEuPathDB; HostDB:ENSG00000155621; -.
DR eggNOG; KOG3241; Eukaryota.
DR GeneTree; ENSGT00440000033805; -.
DR HOGENOM; CLU_218121_0_0_1; -.
DR InParanoid; Q96MD7; -.
DR OMA; KTEHIEN; -.
DR OrthoDB; 1584486at2759; -.
DR PhylomeDB; Q96MD7; -.
DR TreeFam; TF324614; -.
DR PathwayCommons; Q96MD7; -.
DR BioGRID-ORCS; 138241; 30 hits in 1024 CRISPR screens.
DR ChiTaRS; C9orf85; human.
DR GenomeRNAi; 138241; -.
DR Pharos; Q96MD7; Tdark.
DR PRO; PR:Q96MD7; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; Q96MD7; protein.
DR Bgee; ENSG00000155621; Expressed in epithelial cell of pancreas and 176 other tissues.
DR ExpressionAtlas; Q96MD7; baseline and differential.
DR Genevisible; Q96MD7; HS.
DR InterPro; IPR019351; DUF2039.
DR PANTHER; PTHR22876; PTHR22876; 1.
DR Pfam; PF10217; DUF2039; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Phosphoprotein; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CHAIN 2..179
FT /note="Uncharacterized protein C9orf85"
FT /id="PRO_0000089720"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT MOD_RES 137
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q68FU5"
FT VAR_SEQ 35
FT /note="K -> C (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_014428"
FT VAR_SEQ 36..179
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_014429"
FT VAR_SEQ 109..179
FT /note="WSLPLLPRLECSGRILAHHNLRLPCSSDSPASASRVAGTTGAHHHAQLIFVF
FT LVEMGFHYVGQAGLELLTS -> LNKETEKIEHTENNLSSNHRRSCRRNEESDDDLDFD
FT IDLEDTGGDHQMN (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_014430"
FT MOD_RES Q96MD7-1:137
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:19690332"
SQ SEQUENCE 179 AA; 20166 MW; 2DD013912F969A01 CRC64;
MSSQKGNVAR SRPQKHQNTF SFKNDKFDKS VQTKKINAKL HDGVCQRCKE VLEWRVKYSK
YKPLSKPKKC VKCLQKTVKD SYHIMCRPCA CELEVCAKCG KKEDIVIPWS LPLLPRLECS
GRILAHHNLR LPCSSDSPAS ASRVAGTTGA HHHAQLIFVF LVEMGFHYVG QAGLELLTS
