ID   CIA30_CAEEL             Reviewed;         340 AA.
AC   Q18726;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Probable complex I intermediate-associated protein 30, mitochondrial;
DE   AltName: Full=NADH ubiquinone oxidoreductase assembly factor 1;
DE   Flags: Precursor;
GN   Name=nuaf-1; ORFNames=C50B8.3;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=22387847; DOI=10.1016/j.mito.2012.01.003;
RA   van den Ecker D., van den Brand M.A., Ariaans G., Hoffmann M.,
RA   Bossinger O., Mayatepek E., Nijtmans L.G., Distelmaier F.;
RT   "Identification and functional analysis of mitochondrial complex I assembly
RT   factor homologues in C. elegans.";
RL   Mitochondrion 12:399-405(2012).
CC   -!- FUNCTION: Chaperone protein involved in the assembly of the
CC       mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).
CC       Required for normal growth and reproduction.
CC       {ECO:0000269|PubMed:22387847}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown impairs mitochondrial
CC       complex I assembly. Reduced body size, reduced fat content, fewer
CC       embryos and an extended egg laying period. These phenotypes are
CC       exacerbated when nuaf-3 is simultaneously knocked down.
CC       {ECO:0000269|PubMed:22387847}.
CC   -!- SIMILARITY: Belongs to the CIA30 family. {ECO:0000305}.
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DR   EMBL; Z77654; CAB01129.1; -; Genomic_DNA.
DR   PIR; T20097; T20097.
DR   RefSeq; NP_506361.1; NM_073960.3.
DR   AlphaFoldDB; Q18726; -.
DR   SMR; Q18726; -.
DR   BioGRID; 48459; 2.
DR   STRING; 6239.C50B8.3; -.
DR   EPD; Q18726; -.
DR   PaxDb; Q18726; -.
DR   PeptideAtlas; Q18726; -.
DR   PRIDE; Q18726; -.
DR   EnsemblMetazoa; C50B8.3.1; C50B8.3.1; WBGene00008225.
DR   GeneID; 183640; -.
DR   KEGG; cel:CELE_C50B8.3; -.
DR   UCSC; C50B8.3; c. elegans.
DR   CTD; 183640; -.
DR   WormBase; C50B8.3; CE08883; WBGene00008225; nuaf-1.
DR   eggNOG; KOG2435; Eukaryota.
DR   GeneTree; ENSGT00390000007200; -.
DR   HOGENOM; CLU_059028_2_2_1; -.
DR   InParanoid; Q18726; -.
DR   OMA; SMKLEDR; -.
DR   OrthoDB; 1563319at2759; -.
DR   PhylomeDB; Q18726; -.
DR   PRO; PR:Q18726; -.
DR   Proteomes; UP000001940; Chromosome V.
DR   Bgee; WBGene00008225; Expressed in germ line (C elegans) and 4 other tissues.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0051082; F:unfolded protein binding; IBA:GO_Central.
DR   GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; IBA:GO_Central.
DR   GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; IMP:WormBase.
DR   GO; GO:0010257; P:NADH dehydrogenase complex assembly; IBA:GO_Central.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR013857; NADH-UbQ_OxRdtase-assoc_prot30.
DR   InterPro; IPR039131; NDUFAF1.
DR   PANTHER; PTHR13194; PTHR13194; 1.
DR   Pfam; PF08547; CIA30; 1.
DR   SUPFAM; SSF49785; SSF49785; 1.
PE   3: Inferred from homology;
KW   Chaperone; Mitochondrion; Reference proteome; Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..340
FT                   /note="Probable complex I intermediate-associated protein
FT                   30, mitochondrial"
FT                   /id="PRO_0000005466"
SQ   SEQUENCE   340 AA;  38827 MW;  84BF067C31720974 CRC64;
     MITSFPVRNF LPRVTITSCA FFSKESTSAA GTVAEKKESE LQKFAGKSNL LEKKKVGIFG
     TSTVPINTNF PNPKGVVDYD PDFSIKELVA ELPNTRKTQG AKLSEEIKNA FNSVSIEKTE
     LLEDIGFVRH NEARVDYRFD TQEKLDLWKI GCDSDWKEGF STCSLVNSDR GTAVFSGNIS
     TKVLKDGRVE RAGWASMKLE DRKAFNRKKF LSKWRNFSHL LLKVRGDGRS YKIMLHSPLS
     MDFTWGDSFS HPLHTHGGPY WQYEKIPFSK FFHTVAGRIQ DRQYRVNLED TSSIGIVLMD
     RIDGDFRLEI DYIGVYNDTT HVEDFAYETY TLPVFNTHGF