ID   CIAH_STRPN              Reviewed;         444 AA.
AC   P0A4I5; Q54955;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Sensor protein CiaH;
DE            EC=2.7.13.3;
GN   Name=ciaH; OrderedLocusNames=SP_0799;
OS   Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=170187;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-334 / TIGR4;
RX   PubMed=11463916; DOI=10.1126/science.1061217;
RA   Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D.,
RA   Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J.,
RA   Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D.,
RA   Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D.,
RA   Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L.,
RA   McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K.,
RA   Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A.,
RA   Morrison D.A., Hollingshead S.K., Fraser C.M.;
RT   "Complete genome sequence of a virulent isolate of Streptococcus
RT   pneumoniae.";
RL   Science 293:498-506(2001).
CC   -!- FUNCTION: Member of the two-component regulatory system CiaH/CiaR.
CC       Involved in early steps of competence regulation and in penicillin
CC       susceptibility. Probably phosphorylates CiaR (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC         histidine.; EC=2.7.13.3;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}.
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DR   EMBL; AE005672; AAK74936.1; -; Genomic_DNA.
DR   PIR; G95092; G95092.
DR   RefSeq; WP_000491790.1; NZ_AKVY01000001.1.
DR   AlphaFoldDB; P0A4I5; -.
DR   SMR; P0A4I5; -.
DR   STRING; 170187.SP_0799; -.
DR   EnsemblBacteria; AAK74936; AAK74936; SP_0799.
DR   GeneID; 60233461; -.
DR   KEGG; spn:SP_0799; -.
DR   eggNOG; COG5002; Bacteria.
DR   OMA; VMIVFWL; -.
DR   PhylomeDB; P0A4I5; -.
DR   BioCyc; SPNE170187:G1FZB-817-MON; -.
DR   Proteomes; UP000000585; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR   GO; GO:0030420; P:establishment of competence for transformation; IEA:UniProtKB-KW.
DR   CDD; cd00082; HisKA; 1.
DR   Gene3D; 3.30.565.10; -; 1.
DR   InterPro; IPR003594; HATPase_C.
DR   InterPro; IPR036890; HATPase_C_sf.
DR   InterPro; IPR005467; His_kinase_dom.
DR   InterPro; IPR003661; HisK_dim/P.
DR   InterPro; IPR036097; HisK_dim/P_sf.
DR   InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR   Pfam; PF02518; HATPase_c; 1.
DR   Pfam; PF00512; HisKA; 1.
DR   PRINTS; PR00344; BCTRLSENSOR.
DR   SMART; SM00387; HATPase_c; 1.
DR   SMART; SM00388; HisKA; 1.
DR   SUPFAM; SSF47384; SSF47384; 1.
DR   SUPFAM; SSF55874; SSF55874; 1.
DR   PROSITE; PS50109; HIS_KIN; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell membrane; Competence; Kinase; Membrane;
KW   Nucleotide-binding; Phosphoprotein; Transferase; Transmembrane;
KW   Transmembrane helix; Two-component regulatory system.
FT   CHAIN           1..444
FT                   /note="Sensor protein CiaH"
FT                   /id="PRO_0000074722"
FT   TRANSMEM        21..41
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        183..203
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          223..438
FT                   /note="Histidine kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT   MOD_RES         226
FT                   /note="Phosphohistidine; by autocatalysis"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
SQ   SEQUENCE   444 AA;  50673 MW;  1D36CAE8D21D3337 CRC64;
     MFSKLKKTWY ADDFSYFIRN FGVFTLIFST MTLIILQVMH SSLYTSVDDK LHGLSENPQA
     VIQLAINRAT EEIKDLENAR ADASKVEIKP NVSSNTEVIL FDKDFTQLLS GNRFLGLDKI
     KLEKKELGHI YQIQVFNSYG QEEIYRVILM ETNISSVSTN IKYAAVLINT SQLEQASQKH
     EQLIVVVMAS FWILSLLASL YLARVSVRPL LESMQKQQSF VENASHELRT PLAVLQNRLE
     TLFRKPEATI MDVSESIASS LEEVRNMRFL TTSLLNLARR DDGIKPELAE VPTSFFNTTF
     TNYEMIASEN NRVFRFENRI HRTIVTDQLL LKQLMTILFD NAVKYTEEDG EIDFLISATD
     RNLYLLVSDN GIGISTEDKK KIFDRFYRVD KARTRQKGGF GLGLSLAKQI VDALKGTVTV
     KDNKPKGTIF EVKIAIQTPS KKKK