CIAH_STRR6
ID CIAH_STRR6 Reviewed; 444 AA.
AC P0A4I6; Q54955;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=Sensor protein CiaH;
DE EC=2.7.13.3;
GN Name=ciaH; OrderedLocusNames=spr0708;
OS Streptococcus pneumoniae (strain ATCC BAA-255 / R6).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=171101;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8065267; DOI=10.1111/j.1365-2958.1994.tb01038.x;
RA Guenzi E., Gasc A.M., Sicard M.A., Hakenbeck R.;
RT "A two-component signal-transducing system is involved in competence and
RT penicillin susceptibility in laboratory mutants of Streptococcus
RT pneumoniae.";
RL Mol. Microbiol. 12:505-515(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-255 / R6;
RX PubMed=11544234; DOI=10.1128/jb.183.19.5709-5717.2001;
RA Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S.,
RA DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C.,
RA Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J.,
RA Lee L.N., Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M.,
RA McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B.,
RA Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y.,
RA Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R.,
RA Rosteck P.R. Jr., Skatrud P.L., Glass J.I.;
RT "Genome of the bacterium Streptococcus pneumoniae strain R6.";
RL J. Bacteriol. 183:5709-5717(2001).
CC -!- FUNCTION: Member of the two-component regulatory system CiaH/CiaR.
CC Involved in early steps of competence regulation and in penicillin
CC susceptibility. Probably phosphorylates CiaR.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC histidine.; EC=2.7.13.3;
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
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DR EMBL; X77249; CAA54466.1; -; Genomic_DNA.
DR EMBL; AE007317; AAK99512.1; -; Genomic_DNA.
DR PIR; D97960; D97960.
DR PIR; S49545; S49545.
DR RefSeq; NP_358302.1; NC_003098.1.
DR RefSeq; WP_000491790.1; NC_003098.1.
DR AlphaFoldDB; P0A4I6; -.
DR SMR; P0A4I6; -.
DR STRING; 171101.spr0708; -.
DR EnsemblBacteria; AAK99512; AAK99512; spr0708.
DR GeneID; 60233461; -.
DR KEGG; spr:spr0708; -.
DR PATRIC; fig|171101.6.peg.784; -.
DR eggNOG; COG5002; Bacteria.
DR HOGENOM; CLU_000445_89_6_9; -.
DR OMA; VMIVFWL; -.
DR BRENDA; 2.7.13.3; 1960.
DR Proteomes; UP000000586; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR GO; GO:0030420; P:establishment of competence for transformation; IEA:UniProtKB-KW.
DR CDD; cd00082; HisKA; 1.
DR Gene3D; 3.30.565.10; -; 1.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR005467; His_kinase_dom.
DR InterPro; IPR003661; HisK_dim/P.
DR InterPro; IPR036097; HisK_dim/P_sf.
DR InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF00512; HisKA; 1.
DR PRINTS; PR00344; BCTRLSENSOR.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00388; HisKA; 1.
DR SUPFAM; SSF47384; SSF47384; 1.
DR SUPFAM; SSF55874; SSF55874; 1.
DR PROSITE; PS50109; HIS_KIN; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell membrane; Competence; Kinase; Membrane;
KW Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase;
KW Transmembrane; Transmembrane helix; Two-component regulatory system.
FT CHAIN 1..444
FT /note="Sensor protein CiaH"
FT /id="PRO_0000074723"
FT TRANSMEM 21..41
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 183..203
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 223..438
FT /note="Histidine kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT MOD_RES 226
FT /note="Phosphohistidine; by autocatalysis"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
SQ SEQUENCE 444 AA; 50673 MW; 1D36CAE8D21D3337 CRC64;
MFSKLKKTWY ADDFSYFIRN FGVFTLIFST MTLIILQVMH SSLYTSVDDK LHGLSENPQA
VIQLAINRAT EEIKDLENAR ADASKVEIKP NVSSNTEVIL FDKDFTQLLS GNRFLGLDKI
KLEKKELGHI YQIQVFNSYG QEEIYRVILM ETNISSVSTN IKYAAVLINT SQLEQASQKH
EQLIVVVMAS FWILSLLASL YLARVSVRPL LESMQKQQSF VENASHELRT PLAVLQNRLE
TLFRKPEATI MDVSESIASS LEEVRNMRFL TTSLLNLARR DDGIKPELAE VPTSFFNTTF
TNYEMIASEN NRVFRFENRI HRTIVTDQLL LKQLMTILFD NAVKYTEEDG EIDFLISATD
RNLYLLVSDN GIGISTEDKK KIFDRFYRVD KARTRQKGGF GLGLSLAKQI VDALKGTVTV
KDNKPKGTIF EVKIAIQTPS KKKK