CIAO1_DROME
ID CIAO1_DROME Reviewed; 335 AA.
AC Q7K1Y4;
DT 01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Probable cytosolic iron-sulfur protein assembly protein Ciao1 {ECO:0000255|HAMAP-Rule:MF_03037};
GN Name=Ciao1 {ECO:0000255|HAMAP-Rule:MF_03037}; ORFNames=CG12797;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP URMYLATION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=28953965; DOI=10.1371/journal.pone.0185611;
RA Khoshnood B., Dacklin I., Grabbe C.;
RT "A proteomics approach to identify targets of the ubiquitin-like molecule
RT Urm1 in Drosophila melanogaster.";
RL PLoS ONE 12:E0185611-E0185611(2017).
CC -!- FUNCTION: Essential component of the cytosolic iron-sulfur (Fe/S)
CC protein assembly machinery. Required for the maturation of
CC extramitochondrial Fe/S proteins. {ECO:0000255|HAMAP-Rule:MF_03037}.
CC -!- INTERACTION:
CC Q7K1Y4; Q9VTC4: galla-2; NbExp=3; IntAct=EBI-175932, EBI-100085;
CC -!- PTM: Conjugated to URM1, a ubiquitin-like protein.
CC {ECO:0000269|PubMed:28953965}.
CC -!- SIMILARITY: Belongs to the WD repeat CIA1 family. {ECO:0000255|HAMAP-
CC Rule:MF_03037}.
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DR EMBL; AE013599; AAF58195.1; -; Genomic_DNA.
DR EMBL; AY061388; AAL28936.1; -; mRNA.
DR RefSeq; NP_610996.1; NM_137152.4.
DR PDB; 6TBL; X-ray; 2.65 A; E/F=2-335.
DR PDB; 6TBN; X-ray; 2.00 A; B=2-335.
DR PDB; 6TC0; X-ray; 3.60 A; A/D=2-335.
DR PDBsum; 6TBL; -.
DR PDBsum; 6TBN; -.
DR PDBsum; 6TC0; -.
DR AlphaFoldDB; Q7K1Y4; -.
DR SMR; Q7K1Y4; -.
DR BioGRID; 62400; 4.
DR IntAct; Q7K1Y4; 1.
DR STRING; 7227.FBpp0086590; -.
DR PaxDb; Q7K1Y4; -.
DR PRIDE; Q7K1Y4; -.
DR EnsemblMetazoa; FBtr0087460; FBpp0086590; FBgn0033972.
DR GeneID; 36655; -.
DR KEGG; dme:Dmel_CG12797; -.
DR UCSC; CG12797-RA; d. melanogaster.
DR CTD; 9391; -.
DR FlyBase; FBgn0033972; Ciao1.
DR VEuPathDB; VectorBase:FBgn0033972; -.
DR eggNOG; KOG0645; Eukaryota.
DR GeneTree; ENSGT00940000158670; -.
DR HOGENOM; CLU_000288_57_8_1; -.
DR InParanoid; Q7K1Y4; -.
DR OMA; AIWSKSS; -.
DR OrthoDB; 793298at2759; -.
DR PhylomeDB; Q7K1Y4; -.
DR BioGRID-ORCS; 36655; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36655; -.
DR PRO; PR:Q7K1Y4; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0033972; Expressed in crop (Drosophila) and 32 other tissues.
DR Genevisible; Q7K1Y4; DM.
DR GO; GO:0097361; C:CIA complex; IBA:GO_Central.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; ISS:UniProtKB.
DR Gene3D; 2.130.10.10; -; 1.
DR HAMAP; MF_03037; ciao1; 1.
DR InterPro; IPR028608; CIAO1/Cia1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR19920; PTHR19920; 1.
DR Pfam; PF00400; WD40; 6.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 6.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Repeat; Ubl conjugation; WD repeat.
FT CHAIN 1..335
FT /note="Probable cytosolic iron-sulfur protein assembly
FT protein Ciao1"
FT /id="PRO_0000382486"
FT REPEAT 12..51
FT /note="WD 1"
FT REPEAT 57..96
FT /note="WD 2"
FT REPEAT 101..140
FT /note="WD 3"
FT REPEAT 146..185
FT /note="WD 4"
FT REPEAT 192..231
FT /note="WD 5"
FT REPEAT 250..289
FT /note="WD 6"
FT REPEAT 301..335
FT /note="WD 7"
FT STRAND 3..10
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 13..15
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 17..22
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 26..33
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 38..44
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 47..54
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 62..67
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 71..78
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 83..99
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 106..111
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 117..122
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 127..134
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 137..144
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 151..156
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 158..161
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 163..167
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 170..177
FT /evidence="ECO:0007829|PDB:6TBN"
FT TURN 179..181
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 184..191
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 197..202
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 206..213
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 218..224
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 235..238
FT /evidence="ECO:0007829|PDB:6TBL"
FT STRAND 240..247
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 255..260
FT /evidence="ECO:0007829|PDB:6TBN"
FT TURN 262..264
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 267..271
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 276..281
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 287..289
FT /evidence="ECO:0007829|PDB:6TBL"
FT STRAND 292..298
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 301..304
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 306..311
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 313..315
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 318..323
FT /evidence="ECO:0007829|PDB:6TBN"
FT STRAND 328..334
FT /evidence="ECO:0007829|PDB:6TBN"
SQ SEQUENCE 335 AA; 37044 MW; 87A335F8E8966873 CRC64;
MGRLILEHTL QGHKGRIWGV AWHPKGNVFA SCGEDKAIRI WSLTGNTWST KTILSDGHKR
TIREIRWSPC GQYLASASFD ATTAIWSKSS GEFECNATLE GHENEVKSVS WSRSGGLLAT
CSRDKSVWIW EVAGDDEFEC AAVLNPHTQD VKRVVWHPTK DILASASYDN TIKMFAEEPI
DNDWDCTATL TSHTSTVWGI DFDADGERLV SCSDDTTIKI WRAYHPGNTA GVATPDQQTV
WKCVCTVSGQ HSRAIYDVSW CKLTGLIATA CGDDGIRIFK ESSDSKPDEP TFEQITAEEG
AHDQDVNSVQ WNPVVAGQLI SCSDDGTIKI WKVTE
